Low pressure liquid chromatography

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The Niederdruckflüssigkeitschromatographie ( English LPLC Low Pressure Liquid Chromatography, abbreviated ) is a method of liquid chromatography and column chromatography . It is characterized by low pressures of up to approx. 5 bar .

Manual low pressure liquid chromatography

In manual low-pressure liquid chromatography, the hydrostatic pressure of the mixture of substances to be separated and the subsequently refilled solvent is used to drive the mobile phase through the column. The hydrostatic pressure can be up to 2 meters water column . Alternatively, a pump, for example a hose pump, can also be used.

Automated low pressure liquid chromatography

Schematic representation of an automated system for low pressure chromatography

Automated low-pressure systems are used, for example, for the chromatographic separation of liquid substance mixtures in the pharmaceutical , chemical or food industries. The systems usually have automatically controlled inlet groups, one or more pumps , a bubble trap, measurement, control and regulation technology , a switch valve to control the flow direction in the chromatography column and an automatically controlled outlet group. If several pumps are used, gradients or the continuous dilution of concentrated buffers are possible. Different fractions can be collected separately using the sensors integrated in the flow path and the controlled outlet group .

There are commercially available device systems and columns on the market for automated low-pressure liquid chromatography. The order of magnitude ranges from laboratory systems with column volumes of a few milliliters (mL) and flow rates of a few milliliters per minute (mL / min) to industrial systems with flow rates in the range of several cubic meters per hour (m 3 / h) and column diameters up to several Meters. Manufacturers of commercial systems are, for example, Pall , GE Healthcare and Merck Millipore .

literature

  • H. Schmidt-Traub (Ed.): Preparative Chromatography of Fine Chemicals and Pharmaceutical Agents. Wiley-VCH, Weinheim 2005, ISBN 978-3-527-30643-5 .

Individual evidence

  1. ^ Alfred Pingoud , Claus Urbanke: Working methods of biochemistry. Walter de Gruyter, 1997, page 66.