Peptide pattern characterization
The peptide pattern characterization is in pharmaceutical standard laboratory analytical method for identity verification for proteins . In particular, the method is suitable for those proteins which have been produced by DNA recombinant techniques. The method involves chemically or enzymatically treating a protein. The peptide fragments that are formed are then reproducibly separated and then identified.
The peptide pattern characterization allows the recognition of almost every single amino acid exchange, which z. B. occurred due to errors in reading the complementary DNA (cDNA) sequences or point mutations . The method is a comparative procedure, the information obtained is compared with a reference substance treated in an analogous manner . In this way, a possible structural change in the protein under investigation can be recognized and the uniformity of different batches of an industrial production process can be checked.
Individual evidence
- ↑ a b European Pharmacopoeia , Deutscher Apotheker Verlag Stuttgart, 6th edition, 2008, pp. 112–116, ISBN 978-3-7692-3962-1 .