SNA agar
SNA agar (Synthetic nutrient-poor agar or synthetic nutrient-poor agar (SNA)) is a specific to fungi aligned nutrient medium , which in Microbiological is used laboratories. It is a minimal and selective medium that contains macro and micronutrients that are essential for fungi and inhibits the growth of other microorganisms (e.g. bacteria). Since agar has been added, this nutrient medium can be used on Petri dishes to observe the growth of fungal mycelia . Fungal species commonly grown on SNA agar include Penicillium , Fusarium, and Trichoderma .
Components
The culture medium contains glucose , sucrose , potassium hypophosphite , potassium nitrate , potassium chloride , magnesium sulfate and agar. The pH is adjusted to 5.5 with potassium hydroxide solution . The exact quantities vary depending on the manufacturer.
Antibiotics such as chloramphenicol, penicillin G, ampicillin or kanamycin can also be added to the SNA medium in order to effectively inhibit the growth of bacteria.
Incubation and evaluation
SNA agar is normally incubated at 20 ° C and shows growth of fungal colonies that is visible to the eye after one to two days.
Individual evidence
- ↑ Components according to SIFIN, Institute for Immune Preparations and Nutrient Media GmbH Berlin: PDF
swell
- Lecture notes on microbiological exercises SS10, Institute for Environmental Biotechnology, Graz University of Technology