Serum slow agglutination

The Serumlangsamagglutination (SLA) is a serologic method for the detection of antibodies in blood serum and for the indirect detection of infection. It is currently used primarily for the detection of brucellosis and snot in accordance with the official collection of methods of the Friedrich Loeffler Institute .

To do this , a dilution series of the serum sample to be examined is prepared, an antigen dilution is added and the mixture is incubated in a humid chamber at 37 ° C. for 18 to 24 hours. A negative control serum, a serum with a defined antibody titer and an antigen control serve as controls . A positive result is given if a clumping ( agglutination ) forms in the form of an evenly distributed sediment. A negative result shows up as button formation of the antigen. The positive control serum must show a reaction according to the defined titer and serves as a basis of comparison for determining the titer in the sample examined. The formation of buttons must be clearly visible in the negative control serum and in the antigen control.

literature

• Official method collection of the Friedrich Löffler Institute ( online ; PDF; 3.9 MB)

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