Southwestern Blot

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The Southwestern Blot is a biochemical method for determining DNA-protein interactions .

principle

The South Western blotting combines separation of proteins by SDS-PAGE and transfer of proteins to a blotting membrane by Western blotting , followed by the Southern blot borrowed detection of DNA-binding proteins with the help of DNA probes, previously radiolabeled were .

Web links

Individual evidence

  1. ^ B. Bowen, J. Steinberg, UK Laemmli, H. Weintraub: The detection of DNA-binding proteins by protein blotting. In: Nucleic acids research. Volume 8, Number 1, January 1980, pp. 1-20, PMID 6243775 , PMC 327239 (free full text).
  2. ↑ C. Franke, D. Gräfe, H. Bartsch, M. Bachmann: Use of non-radioactive detection method for north and southwestern blot. In: Methods in molecular biology (Clifton, NJ). Volume 536, 2009, pp. 441-449, doi : 10.1007 / 978-1-59745-542-8_44 , PMID 19378081 .
  3. ^ IM Rosenberg: Protein Analysis and Purification. Springer Science & Business Media, 2013, ISBN 978-1-475-71108-0 , p. 169.