TBE buffer

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TBE buffer is the abbreviation for T RIS- B orat- E DTA buffer, one according to its components

named electrophoresis buffer . TBE buffers may be used. a. used in agarose gel electrophoresis . The concentration of the buffer used varies from laboratory to laboratory. As a rule, 0.5-fold or 1-fold concentrated TBE buffers are used. The TRIS and boric acid concentrations are mostly both the same and between 50 and 90 millimolar with a single concentrated TBE buffer . The EDTA concentration is between 1 and 2 mM. The pH value is usually set to a value between 8 and 8.9.

For the production of a TBE buffer (1-fold) pH 8.0 z. B. 10.8 g (89 mM) TRIS base, 5.5 g (89 mM) boric acid and 0.7 g (2 mM) EDTA-Na 2 made up to 1000 ml with distilled (demineralized) water.

Alternatively used electrophoresis buffers are e.g. B. the TAE buffer , the TPE buffer , SB buffer and the LB buffer . Compared to TAE buffers, TBE buffers have a higher buffer capacity. However, it also tends to be more heated, which means that somewhat lower voltages must be applied to the gel. The migration speed is about half as high as with TAE buffer. Concentrated stock solutions of TBE buffers tend to precipitate the Tris-borate, which can result in inhomogeneous buffer compositions.

Individual evidence

  1. ^ Joseph Sambrook , David W. Russell: Molecular Cloning. A laboratory manual. 3 volumes. 3rd edition. Cold Spring Harbor Laboratory Press, Cold Spring Harbor NY 2001, ISBN 0-87969-577-3 .
  2. ^ A b Gerard Meurant: Human Molecular Genetics. Academic Press, 1996, ISBN 978-0-080-53641-5 , p. 285.
  3. ↑ a b Wafik S. El-Deiry: Tumor Suppressor Genes. Springer Science & Business Media, 2003, ISBN 978-1-592-59329-3 , p. 93.
  4. ^ A b Didier Picard: Nuclear Receptors: A Practical Approach. OUP Oxford, 1999, ISBN 978-0-191-56592-2 , p. 47.
  5. ↑ a b c d Lela Buckingham: Molecular Diagnostics. FA Davis, 2011, ISBN 978-0-803-62975-2 . P. 95.