TAE buffer

from Wikipedia, the free encyclopedia

TAE buffer is the abbreviation for T RIS A cetat- E DTA buffer one by its ingredients

named electrophoresis buffer . TAE buffers are u. a. used in agarose gel electrophoresis to separate nucleic acids . The concentrations for tris and acetic acid are mostly between 40 and 50 millimolar . The EDTA concentration is 1 mM. The pH value is usually set to 8.

Alternatively used buffers for separating nucleic acids are e.g. B. TBE buffer , TPE buffer , SB buffer or LB buffer . Compared to TBE and TPE buffers, TAE buffer is heated less during electrophoresis, which allows higher voltages to be applied to the gel. However, it has a smaller buffer capacity than a TBE buffer. The migration speed in TAE buffers is twice as high as in a TBE buffer.

Individual evidence

  1. ^ Joseph Sambrook , David W. Russell: Molecular Cloning. A laboratory manual. 3 volumes. 3rd edition. Cold Spring Harbor Laboratory Press, Cold Spring Harbor NY 2001, ISBN 0-87969-577-3 .
  2. ^ A b Ralph Rapley: The Nucleic Acid Protocols Handbook. Springer Science & Business Media, 2008, ISBN 978-1-592-59038-4 , p. 707.
  3. ↑ a b Minou Bina: Gene Mapping, Discovery, and Expression. Springer Science & Business Media, 2006, ISBN 978-1-597-45097-3 , p. 262.
  4. ↑ a b Monika Jansohn: genetic engineering methods . Springer-Verlag, 2012, ISBN 978-3-827-42430-3 . P. 125.
  5. ^ A b c Lela Buckingham: Molecular Diagnostics. FA Davis, 2011, ISBN 978-0-803-62975-2 . P. 95.