Sodium borate buffer

Sodium borate buffer ( SB buffer ) is an electrophoresis buffer that is used in biochemistry and molecular biology in the course of agarose gel electrophoresis to separate nucleic acids such as DNA or RNA .

properties

The SB buffer is composed of sodium hydroxide and boric acid (1–10 mM , pH 8.0) and, compared to the alternative buffers ( TAE buffer , TBE buffer , TPE buffer ), has a lower ionic strength and therefore a lower electrical conductivity , only surpassed by the lithium borate buffer . This allows a higher applied electrical voltage (5-35 V / cm gel length compared to 5-10 V / cm) and therefore a higher migration speed of the nucleic acids without heating the agarose gel . This shortens the time required for electrophoretic separation.

Web links

• Sodium borate as an electrophoresis buffer

Individual evidence

1. ↑ Jonathan R. Brody, Scott E. Kern: Sodium boric acid: a tris-free, cooler conductive medium for DNA electrophoresis. In: BioTechniques. Vol. 36, No. 2, 2004, ISSN  0736-6205 , pp. 214–215, PMID 14989083 (PDF; 155 kB) ( Memento of the original from July 24, 2011 in the Internet Archive ) Info: The archive link was automatically inserted and not yet checked. Please check the original and archive link according to the instructions and then remove this notice. . 
2. ↑ Jonathan R. Brody, Eric S. Calhoun, Eike Gallmeier, Talisa D. Creavalle, Scott E. Kern (2004): Ultra-fast high-resolution agarose electrophoresis of DNA and RNA using low-molarity conductive media. ( Memento of the original of August 14, 2017 in the Internet Archive ) Info: The archive link was automatically inserted and not yet checked. Please check the original and archive link according to the instructions and then remove this notice. In: BioTechniques. Vol. 37, No. 4, 2004, pp. 598–602, PMID 15517972 (PDF; 198 kB)  ( page no longer available , search in web archives )  Info: The link was automatically marked as defective. Please check the link according to the instructions and then remove this notice. .   
3. ↑ Jonathan R. Brody, Scott E. Kern: History and principles of conductive media for standard DNA electrophoresis. In: Analytical Biochemistry . Vol. 333, No. 1, 2004, pp. 1-13, PMID 15351274 (PDF; 1.14 MB) .