Tris-glycine buffer

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Tris-glycine buffer, also known as Laemmli buffer, is an electrophoresis buffer that is used in biochemistry and molecular biology in the course of SDS-PAGE to separate proteins.

properties

The Tris-glycine buffer ( pH 8.3) is composed of TRIS (25 mM ), glycine (192 mM) and SDS (0.1% w / v).

Alternatively used buffers are the TRIS-Tricine (pH 8.24) from TRIS (50 mM), Tricine (50 mM), EDTA (1 mM) and SDS (0.1% m / V), the Tris-MOPS- (pH 7.7) from TRIS (50 mM), MOPS (50 mM), EDTA (1 mM) and SDS (0.1% m / V) and the Tris-MES buffer system (pH 7.3) from TRIS (50 mM), MES (50 mM), EDTA (1 mM) and SDS (0.1% w / v). The Tris-MOPS and Tris-MES buffer systems are used in particular for commercially available gels which are stabilized by the neutral pH value.

The Tris-Glycine buffer is based on the buffer for discontinuous electrophoresis by L. Ornstein and BJ Davis.

Web links

Individual evidence

  1. ^ UK Laemmli: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. In: Nature. Volume 227, Number 5259, August 1970, pp. 680-685, PMID 5432063 .
  2. ↑ H. Schägger: Tricine-SDS-PAGE. In: Nature protocols. Volume 1, number 1, 2006, pp. 16-22, doi : 10.1038 / nprot.2006.4 , PMID 17406207 .
  3. ↑ Hermann Schägger, Gebhard von Jagow: Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. In: Analytical Biochemistry . Vol. 166, 1987, pp. 368-379, doi : 10.1016 / 0003-2697 (87) 90587-2 , PMID 2449095 .
  4. ↑ L. Ornstein, BJ Davis: Disc Electrophoresis -1. Background and Theory. In: Ann NY Acad Sci. Volume 121, 1964, pp. 321-349.