N - (tri (hydroxymethyl) methyl) glycine

Structural formula
General
Surname	N- (tri (hydroxymethyl) methyl) glycine
other names	Tricine; N - (tri (hydroxymethyl) methyl) glycine; N - [2-Hydroxy-1,1-bis (hydroxymethyl) ethyl] glycine; 2 - [{1,3-dihydroxy-2- (hydroxymethyl) propan-2-yl} amino] acetic acid;
Molecular formula	C 6 H 13 NO 5
Brief description	colorless crystals
External identifiers / databases
EC number	227-193-6
ECHA InfoCard	100,024,721
PubChem	79784
ChemSpider	72078
Wikidata	Q409516
properties
Molar mass	179.17 g mol −1
Physical state	firmly
Melting point	187 ° C
pK s value	pK s1 : 2.3; pK s2 : 8.15 (20 ° C);
solubility	0.8 mol / l in water at 0 ° C
safety instructions
GHS labeling of hazardous substances	no GHS pictograms
	no GHS pictograms
H and P phrases	H: no H-phrases
	P: no P-phrases
	As far as possible and customary, SI units are used. Unless otherwise noted, the data given apply to standard conditions .

N - (Tri (hydroxymethyl) methyl) glycine , also tricine , is an organic chemical compound from the group of aminocarboxylic acids and polyols .

properties

Tricine is a zwitterion with a pK _s1 of 2.3 and a pK _s2 of 8.6 (0 ° C), 8.15 (20 ° C), 8.05 (25 ° C) and 7.80 (37 ° C). The temperature dependence of the acid constant is ΔpK _s2 = −0.021 K ⁻¹ .

Tricine binds divalent metal ions with the following equilibrium constants (pK M at 20 ° C.): Mg ²⁺ 1.2; Ca ²⁺ 2.4; Mn ²⁺ 2.7; Cu ²⁺ 7.3.

use

Tricine is used for biochemical purposes as a buffer substance from the group of Good buffers , with a good buffer capacity between pH 7.4-8.8.

Tricine is used in TRIS tricine gels in SDS-PAGE .

Individual evidence

↑ ^a ^b Entry on N- [Tris (hydroxymethyl) methyl] glycine. Template: Linktext-Check / EscapedIn: Römpp Online . Georg Thieme Verlag, accessed on June 20, 2014.
↑ ^a ^b ^c ^d ^e ^f N. E. Good et al: Hydrogen Ion Buffers for Biological Research. In: Biochemistry. Vol. 5, No. 2 (1966), pp. 467-477. doi : 10.1021 / bi00866a011 .
↑ ^a ^b Data sheet N- (Tri (hydroxymethyl) methyl) glycine from Sigma-Aldrich , accessed on June 15, 2011 ( PDF ).

[Römpp-1] Entry on N- [Tris (hydroxymethyl) methyl] glycine. Template: Linktext-Check / EscapedIn: Römpp Online . Georg Thieme Verlag, accessed on June 20, 2014.

[Good-2] ↑ ^a ^b ^c ^d ^e ^f N. E. Good et al: Hydrogen Ion Buffers for Biological Research. In: Biochemistry. Vol. 5, No. 2 (1966), pp. 467-477. doi : 10.1021 / bi00866a011 .

[Sigma-3] Data sheet N- (Tri (hydroxymethyl) methyl) glycine from Sigma-Aldrich , accessed on June 15, 2011 ( PDF ).