Vanillin HCl staining

Vanillin

The vanillin-HCl staining is a histological technique for coloring tannins with a vanillin - hydrochloric acid solution.

properties

The vanillin HCl staining solution consists of ten percent vanillin in an ethanol hydrochloric acid solution. Tannins are colored orange-red in the vanillin-HCl coloring. In this way, among other things, the localization of the phlorotannins can be determined. Proanthocyanidins or catechins are colored red with the vanillin HCl stain.

A variant of the vanilla coloring (vanillin with sulfuric acid ) is used in thin layer chromatography to detect unsaturated lipids .

Individual evidence

^ RO Gardner: Vanillin-hydrochloric acid as a histochemical test for tannin. In: Stain technology. Volume 50, Number 5, September 1975, pp. 315-317, PMID 54955 .
^ L. Pellegrini: Cytological studies on physodes in the vegetative cells of Cystoseira stricta Sauvageau (Phaeophyta, Fucales). In: Journal of cell science. Volume 41, February 1980, pp. 209-231, PMID 7364883 .

[1] RO Gardner: Vanillin-hydrochloric acid as a histochemical test for tannin. In: Stain technology. Volume 50, Number 5, September 1975, pp. 315-317, PMID 54955 .

[2] L. Pellegrini: Cytological studies on physodes in the vegetative cells of Cystoseira stricta Sauvageau (Phaeophyta, Fucales). In: Journal of cell science. Volume 41, February 1980, pp. 209-231, PMID 7364883 .