Zone electrophoresis

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The zone electrophoresis is a classic electrophoresis and allows separations of charged or chargeable particles in a homogeneous buffer system with a constant pH by an electric field . It differs from discontinuous electrophoresis through the zone with a homogeneous pH value .

principle

Schematic representation of zone electrophoresis

The separation takes place according to the electrophoretic mobility (synonymous electrophoretic mobility , defined as the migration speed of particles in an electric field over a period of time) and the charge density . The distance covered by the analytes in the separation medium within a defined period and a defined mobile phase is a measure of this mobility. The more an analyte is charged, the further it migrates in the electric field. The mobility of the analytes therefore depends on the type and level of the charge, the hydrodynamic radius and sometimes the structure .

Applications

Zone electrophoresis is used primarily as an analytical method in biology. Here the process is mainly used by means of free-flow electrophoresis for the separation and purification of subcellular particles such as organelles (e.g. mitochondria and ribosomes ).

Individual evidence

  1. ^ RT Turgeon, MT Bowser: Micro free-flow electrophoresis: theory and applications. In: Anal Bioanal Chem. (2009), Volume 394, Issue 1, pp. 187-198. doi : 10.1007 / s00216-009-2656-5 . PMID 19290514 ; PMC 2739039 (free full text).
  2. ↑ H. Zischka, N. Kinkl, RJ Braun, M. Ueffing: Purification of Saccharomyces cerevisiae mitochondria by zone electrophoresis in a free flow device. In: Methods Mol Biol. (2008), Volume 432, pp. 51-64. doi : 10.1007 / 978-1-59745-028-7_4 . PMID 18370010 .
  3. ↑ M. Islinger, A. Abdolzade-Bavil, p Liebler, G. Weber, A. Völkl: Assessing heterogeneity of peroxisomes: isolation of two subpopulations from rat liver. In: Methods in Molecular Biology (2012), Volume 909, pp. 83-96. doi : 10.1007 / 978-1-61779-959-4_6 . PMID 22903710 .
  4. ↑ X. Subirats, D. Blaas, E. Kenndler: Recent developments in capillary and chip electrophoresis of bioparticles: Viruses, organelles, and cells. In: Electrophoresis (2011), Volume 32, Issue 13, pp. 1579-1590. doi : 10.1002 / elps.201100048 . PMID 21647924 .