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[[File:1ppr peridinin chlorophyll protein.png|thumb|right|[[Crystal structure]] of the soluble peridinin-chlorophyll-protein complex from the photosynthetic dinoflagellate ''[[Amphidinium carterae]]''. This complex is found in many photosynthetic dinoflagellates and involves a boat or cradle-shaped protein with two pseudosymmetrical repeats of eight alpha helices (shown in blue and orange) wrapped around a pigment-filled central cavity. Each eight-helix segment binds one chlorophyll molecule (green, with central magnesium ion shown as a green sphere), one diacylglycerol molecule (yellow) and four peridinin molecules (gray).<ref name=hofmann_1996 />]] |
[[File:1ppr peridinin chlorophyll protein.png|thumb|right|[[Crystal structure]] of the soluble peridinin-chlorophyll-protein complex from the photosynthetic dinoflagellate ''[[Amphidinium carterae]]''. This complex is found in many photosynthetic dinoflagellates and involves a boat or cradle-shaped protein with two pseudosymmetrical repeats of eight alpha helices (shown in blue and orange) wrapped around a pigment-filled central cavity. Each eight-helix segment binds one chlorophyll molecule (green, with central magnesium ion shown as a green sphere), one diacylglycerol molecule (yellow) and four peridinin molecules (gray).<ref name=hofmann_1996 />]] |
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Peridinin is an [[apocarotenoid]] [[pigment]] that some organisms use in [[photosynthesis]]. Many photosynthetic [[dinoflagellate]]s use peridinin, which absorbs blue-green light in the 470-550nm range, outside the range accessible to [[chlorophyll]] molecules. The [[peridinin-chlorophyll-protein complex]] is a specialized molecular complex consisting of a boat-shaped protein molecule with a large central cavity that contains peridinin, chlorophyll, and lipid molecules, usually in a 4:1 ratio of peridinin to chlorophyll.<ref name="hofmann_1996">{{cite journal|last1=Hofmann|first1=E|last2=Wrench|first2=PM|last3=Sharples|first3=FP|last4=Hiller|first4=RG|last5=Welte|first5=W|last6=Diederichs|first6=K|title=Structural basis of light harvesting by carotenoids: peridinin-chlorophyll-protein from Amphidinium carterae.|journal=Science|date=21 June 1996|volume=272|issue=5269|pages=1788–91|pmid=8650577|doi=10.1126/science.272.5269.1788}}</ref><ref name="schulte_2010">{{cite journal|last1=Schulte|first1=Tim|last2=Johanning|first2=Silke|last3=Hofmann|first3=Eckhard|title=Structure and function of native and refolded peridinin-chlorophyll-proteins from dinoflagellates|journal=European Journal of Cell Biology|date=December 2010|volume=89|issue=12|pages=990–997|doi=10.1016/j.ejcb.2010.08.004|pmid=20846743}}</ref><ref name="jiang_2012">{{cite journal|last1=Jiang|first1=Jing|last2=Zhang|first2=Hao|last3=Kang|first3=Yisheng|last4=Bina|first4=David|last5=Lo|first5=Cynthia S.|last6=Blankenship|first6=Robert E.|title=Characterization of the peridinin–chlorophyll a-protein complex in the dinoflagellate Symbiodinium|journal=Biochimica et Biophysica Acta (BBA) - Bioenergetics|date=July 2012|volume=1817|issue=7|pages=983–989|doi=10.1016/j.bbabio.2012.03.027|pmid=22497797}}</ref> |
Peridinin is an [[apocarotenoid]] [[pigment]] that some organisms use in [[photosynthesis]]. Many photosynthetic [[dinoflagellate]]s use peridinin, which absorbs blue-green light in the 470-550nm range, outside the range accessible to [[chlorophyll]] molecules. The [[peridinin-chlorophyll-protein complex]] is a specialized molecular complex consisting of a boat-shaped protein molecule with a large central cavity that contains peridinin, chlorophyll, and lipid molecules, usually in a 4:1 ratio of peridinin to chlorophyll.<ref name="hofmann_1996">{{cite journal|last1=Hofmann|first1=E|last2=Wrench|first2=PM|last3=Sharples|first3=FP|last4=Hiller|first4=RG|last5=Welte|first5=W|last6=Diederichs|first6=K|title=Structural basis of light harvesting by carotenoids: peridinin-chlorophyll-protein from Amphidinium carterae.|journal=Science|date=21 June 1996|volume=272|issue=5269|pages=1788–91|pmid=8650577|doi=10.1126/science.272.5269.1788}}</ref><ref name="schulte_2010">{{cite journal|last1=Schulte|first1=Tim|last2=Johanning|first2=Silke|last3=Hofmann|first3=Eckhard|title=Structure and function of native and refolded peridinin-chlorophyll-proteins from dinoflagellates|journal=European Journal of Cell Biology|date=December 2010|volume=89|issue=12|pages=990–997|doi=10.1016/j.ejcb.2010.08.004|pmid=20846743}}</ref><ref name="jiang_2012">{{cite journal|last1=Jiang|first1=Jing|last2=Zhang|first2=Hao|last3=Kang|first3=Yisheng|last4=Bina|first4=David|last5=Lo|first5=Cynthia S.|last6=Blankenship|first6=Robert E.|title=Characterization of the peridinin–chlorophyll a-protein complex in the dinoflagellate Symbiodinium|journal=Biochimica et Biophysica Acta (BBA) - Bioenergetics|date=July 2012|volume=1817|issue=7|pages=983–989|doi=10.1016/j.bbabio.2012.03.027|pmid=22497797|pmc=3947849}}</ref> |
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==Spectral characteristics== |
==Spectral characteristics== |
Revision as of 17:29, 23 July 2019
Names | |
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IUPAC name
Acetic acid [(1S,3R)-3-hydroxy-4-[(3E,5E,7E,9E,11Z)-11-[4-[(E)-2-[(1S,4S,6R)-4-hydroxy-2,2,6-trimethyl-7-oxabicyclo[4.1.0]heptan-1-yl]vinyl]-5-oxo-2-furylidene]-3,10-dimethylundeca-1,3,5,7,9-pentaenylidene]-3,5,5-trimethylcyclohexyl] ester
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Identifiers | |
3D model (JSmol)
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PubChem CID
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CompTox Dashboard (EPA)
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Properties | |
C39H50O7 | |
Molar mass | 630.822 g·mol−1 |
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
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Peridinin is a light-harvesting apocarotenoid, a pigment associated with chlorophyll and found in the peridinin-chlorophyll-protein (PCP) light-harvesting complex in dinoflagellates, best studied in Amphidinium carterae.[1]
Biological significance
Peridinin is an apocarotenoid pigment that some organisms use in photosynthesis. Many photosynthetic dinoflagellates use peridinin, which absorbs blue-green light in the 470-550nm range, outside the range accessible to chlorophyll molecules. The peridinin-chlorophyll-protein complex is a specialized molecular complex consisting of a boat-shaped protein molecule with a large central cavity that contains peridinin, chlorophyll, and lipid molecules, usually in a 4:1 ratio of peridinin to chlorophyll.[2][3][4]
Spectral characteristics
- Absorption maximum: 483 nm<
- Emission maximum: 676 nm
- Extinction coefficient (ε): 1.96 x 106 M−1cm−1
- A483/A280 ≥ 4.6
Applications
Peridinin chlorophyll (PerCP) is commonly used in immunoassays such as fluorescence-activated cell sorting (FACS) and flow cytometry. The fluorophore is covalently linked to proteins or antibodies for use in research applications.[5]
References
- ^ Hofmann E, Wrench PM, Sharples FP, Hiller RG, Welte W, Diederichs K (1996). "Structural basis of light harvesting by carotenoids: peridinin-chlorophyll-protein from Amphidinium carterae". Science. 272 (5269): 1788–1791. doi:10.1126/science.272.5269.1788. PMID 8650577.
- ^ a b Hofmann, E; Wrench, PM; Sharples, FP; Hiller, RG; Welte, W; Diederichs, K (21 June 1996). "Structural basis of light harvesting by carotenoids: peridinin-chlorophyll-protein from Amphidinium carterae". Science. 272 (5269): 1788–91. doi:10.1126/science.272.5269.1788. PMID 8650577.
- ^ Schulte, Tim; Johanning, Silke; Hofmann, Eckhard (December 2010). "Structure and function of native and refolded peridinin-chlorophyll-proteins from dinoflagellates". European Journal of Cell Biology. 89 (12): 990–997. doi:10.1016/j.ejcb.2010.08.004. PMID 20846743.
- ^ Jiang, Jing; Zhang, Hao; Kang, Yisheng; Bina, David; Lo, Cynthia S.; Blankenship, Robert E. (July 2012). "Characterization of the peridinin–chlorophyll a-protein complex in the dinoflagellate Symbiodinium". Biochimica et Biophysica Acta (BBA) - Bioenergetics. 1817 (7): 983–989. doi:10.1016/j.bbabio.2012.03.027. PMC 3947849. PMID 22497797.
- ^ "Peridinin Chlorophyll (PerCP)".