Impression preparation
A contact specimen is the removal of a sample from a biological surface, which is applied to a slide and then examined directly under the microscope - native or after staining . Impression preparations are primarily produced in dermatology and oncology and enable quick and inexpensive diagnostics.
To take samples, the slide itself can be pressed onto the area to be examined (for example, an ulcerated skin lesion or a tumor incision ); in the case of heavily exuding lesions, it is advisable to carefully dab it dry beforehand. Rotating or wiping movements should be avoided when taking samples, as they can lead to cell destruction. Alternatively, the impression can be made indirectly with a transparent adhesive strip . The latter is mainly used for diagnosing superficial, mobile parasites such as predatory mites or for the detection of bacteria , dermatophytes and yeasts in skin lesions. The adhesive strip is then applied to a slide, if necessary after staining.
Individual evidence
- ↑ Martin Kessler (ed.): Small animal oncology: diagnosis and therapy of tumor diseases in dogs and cats . 3. Edition. Georg Thieme, Stuttgart 2012, ISBN 978-3-8304-1137-6 , p. 62 .
- ↑ Reinhard Mischke: Cytological internship for veterinary medicine . Schlütersche, Hannover 2005, ISBN 978-3-89993-013-9 , p. 27 .