Affinity magnetic separation
The affinity Magnetic Separation ( AMS , also Magnetic affinity separation called), is a laboratory method by which bacteria can be removed effectively from body fluids or cell cultures. It can also be used to quantify the pathogenicity of blood, food, or feces. Another separation method is immunomagnetic separation (IMS), which is more suitable for isolating eukaryotic cells.
technology
Bacteriophages recognize their hosts through bacteria-binding proteins at the tail ends , which have a strong affinity for proteins or hydrocarbon structures on the host surface. Paramagnetic beads coated with these phage proteins bind specific cell components on the host surface and thereby capture these cells (phage ligand technology). A magnet is then attached to the outside of the test vessel, which attracts the cells bound to the beads and thereby concentrates them.
swell
- ↑ J. Kretzer, R. Grassl, M. Biebl, S. Miller: Use of bacteriophage proteins for the specific separation of Escherichia coli O157 from food. Stuttgart: 10th symposium of the DGHM specialist group food microbiology, 2008.
- ↑ C. Rozand, PCH Feng: Specificity analysis of a novel Phage-derived ligand in an Enzyme-Linked Fluorescent Assay for the detection of Escherichia coli O157: H7. In: Journal of Food Protection. 72, 2009, pp. 1078-1081.