Alcian blue staining

from Wikipedia, the free encyclopedia
Alcian blue staining of a histological thin section of a mucous membrane , especially the goblet cells

The Alcian blue staining is a histological staining method, when Alcian blue for coloring acidic proteoglycans and polysaccharides is used.

properties

Alcian blue colors acid mucins and sulfated glycosaminoglycans (such as hyaluronic acid or chondroitin sulfate ) selectively cyan at a low pH value (1.0 to 2.5) . With increasing ionic strength , coloring with Alcian blue is prevented, whereby a characteristic salt concentration ( critical electrolyte concentration , CEC) can be determined at which coloring no longer takes place.

In histology, the derivative Alcian blue 8G (formerly 8GX, different production) is mainly used. Alternatively, staining with zirconyl hematoxylin can be carried out. In combination with alizarin red , Alcian blue is used to stain bone and cartilage tissue. A PAS staining can be performed with Alcian before staining to neutral mucins, neutral glycoproteins and glycogen magenta coloring. The staining with Alcian blue can also be combined with the hematoxylin-eosin staining and the Van Gieson staining .

history

It was introduced into histology in 1950 by Harold F. Steedman as the "mucus dye". It has become one of the most important dyes in histology.

literature

  • JE Scott: Alcian blue. Now you see it, now you don't. In: European journal of oral sciences. Volume 104, Number 1, February 1996, pp. 2-9, PMID 8653492 .

Individual evidence

  1. ↑ a b c d John D. Bancroft: Theory and Practice of Histological Techniques. Elsevier Health Sciences, 2008, ISBN 978-0-443-10279-0 , p. 172.
  2. ^ W. Dong, YK Matsuno, A. Kameyama: A procedure for Alcian blue staining of mucins on polyvinylidene difluoride membranes. In: Analytical chemistry. Volume 84, Number 20, October 2012, pp. 8461-8466, doi : 10.1021 / ac301678z , PMID 22950532 .
  3. ^ University of Zurich: Histology course, online here
  4. ↑ Pathology Institute of the LMU: Histological staining, here online
  5. ↑ Data sheet ALCIANBLAU 8 GS (CI 74240) (PDF) from Carl Roth , accessed on March 13, 2016.
  6. ↑ Jürgen Bruns: Tissue Engineering News on tissue replacement in the musculoskeletal system . Springer-Verlag, 2011, ISBN 978-3-642-57353-8 , pp. 68 ( limited preview in Google Book search).
  7. ↑ a b M.A. Hayat: Stains and Cytochemical Methods . Springer Science & Business Media, 1993, ISBN 978-0-306-44294-0 , pp. 80 ( limited preview in Google Book search).
  8. ↑ SE Batran, YM Zakout: Evaluation of improved zirconyl hematoxylin compared to the classical pH 2.5 Alcian blue method for demonstrating acid mucins. In: Biotechnic & histochemistry: official publication of the Biological Stain Commission. Volume 87, Number 3, April 2012, pp. 226-228, doi : 10.3109 / 10520295.2011.608717 , PMID 21955130 .
  9. ↑ D. Ovchinnikov: Alcian blue / alizarin red staining of cartilage and bone in mouse. In: Cold Spring Harbor protocols. Volume 2009, number 3, March 2009, p. Pdb.prot5170, doi : 10.1101 / pdb.prot5170 , PMID 20147105 .
  10. ↑ Barbara Young (Ph. D.): Wheater's Functional Histology. Elsevier Health Sciences, 2006, ISBN 978-0-443-06850-8 , p. 428.
  11. ↑ W. Mohr: Pathology of the ligament apparatus tendons · tendon sheaths · fascia · bursa . Springer-Verlag, 2013, ISBN 978-3-642-72843-3 , p. 37 ( limited preview in Google Book search).
  12. ↑ HF Steedman: Alcian Blue 8GS: A New Stain for Mucin . In: Quarterly Journal of Microscopical Science . tape 91 , 1950, pp. 477-479 , PMID 24540170 ( PDF ).