Batch culture ( syn. Batch, batch , static or discontinuous culture ) denotes a batch process for the culture (reproduction) of microorganisms . The microorganisms are introduced into a culture medium . The medium remains the same during the entire culture period, is neither supplemented nor filled up at times ( fed-batch process ), nor is it partially replaced ( chemostat bioreactor ).
The growth of the microorganism population , defined as an increase in microorganisms number in a batch culture typically rises after initial Constance (deceleration phase, English lag-phase ) very strongly ( exponential phase ), then takes more and more from (transitional phase) suspended in conclusion (stationary phase) and possibly also becomes negative (death phase). Depletion of the nutrient medium and the accumulation of toxic substances are seen as the cause of the stagnation in growth.
- Oliver Kayser: Basic knowledge of pharmaceutical biotechnology. Teubner, Wiesbaden 2002, ISBN 3-519-03553-7 , p. 82.
- Gerhard Richter: Practical Biochemistry. Thieme, Stuttgart 2003, ISBN 3-13-132381-7 , p. 7.