CHO cells

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Adherent CHO cells in a cell culture bottle ( phase contrast ).
Greater magnification.

As CHO cells , abbreviated by English Chinese Hamster Ovary is an immortalized cell line from ovaries of the Chinese hamster dwarf ( Cricetulus griseus hereinafter) which in the cell biology and biotechnology for the production of recombinant proteins is used. There are different cell lines with different genetic changes.

The immortalized cell line was originally isolated in 1957 by Theodore T. Puck from a primary culture of ovarian cells of a Chinese hamster.

The CHO cell line is one of the most frequently used cell lines in the biotechnological production of active substances such as therapeutic antibodies . Almost 70% of all proteins produced recombinantly in cultured mammalian cells and intended for therapeutic use are now expressed in CHO cells.

literature

  1. ↑ HJ Tjio, TT Puck (1958): Genetics of somatic mammalian cells. II. Chromosomal constitution of cells in tissue culture. In: J. Exp. Med. 108 (2): 259-271. PMID 13563760 doi : 10.1084 / jem.108.2.259
  2. ↑ TT Puck, SJ Cieciura, A. Robinson (1958): Genetics of somatic mammalian cells. III. Long-term cultivation of euploid cells from human and animal subjects. In: J. Exp. Med. 108 (6): 945-956. PMID 13598821 doi : 10.1084 / jem.108.6.945
  3. ↑ KP Jayapal, KF Wlaschin, MGS Yap, WS. Hu (2007): Recombinant protein therapeutics from CHO cells - 20 years and counting. In: Chem. Eng. Prog. 103 (7): 40-47. PDF
  4. ↑ DL Hacker, M. De Jesus, FM Wurm (2009): 25 years of recombinant proteins from reactor-grown cells - where do we go from here? In: Biotechnol. Adv. 27 (6): 1023-1027. PMID 19463938 doi : 10.1016 / j.biotechadv.2009.05.008
  5. ↑ FM Wurm (2004): Production of recombinant protein therapeutics in cultivated mammalian cells. In: Nat. Biotechnol. 22 (11): 1393-1398. PMID 15529164

Web links

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