Cap binding complex
| Cap binding complex | ||
|---|---|---|
|
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| CBC surface model seen from two sides (long chain pink, short yellow, m7G and GDP as domes), according to PDB 1H2T | ||
| Mass / length primary structure | 790/156 amino acids | |
| Identifier | ||
| Gene name (s) | NCBP1 , NCBP2 | |
| External IDs | ||
| Occurrence | ||
| Parent taxon | multicellular animals | |
The cap binding complex (CBC), more precisely the nuclear cap binding complex (NCBC), is a protein complex consisting of two subunits that binds to the 5'-cap structure of the resulting mRNA in the nucleus of eukaryotes . The binding of CBC stabilizes the RNA and protects it from the removal of the m 7 G-Cap; in addition, after the end of the transcription, it leads to the poly-A tail entering into a bond, and so the RNA is cyclized and offers even less surface to attack. Furthermore, the CBC is indispensable for the process of splicing and transport from the cell nucleus to the endoplasmic reticulum . The CBC in metazoa consists of the subunits CBP20 and CBP80, the naming was based on the molecular mass of 20 kDa and 80 kDa, respectively. In yeast the subunits are called CBC1 and CBC2.
In addition to the functions mentioned, mRNA is protected from degradation by poly-A-specific ribonuclease by binding to CBC even during the first round of translation .
Individual evidence
- ↑ Homologues at OMA
- ↑ UniProt P52298 , UniProt Q09161
- ↑ Buratowski / reactome: Recognition and binding of the mRNA cap by the cap-binding complex ( page no longer available , search in web archives ) Info: The link was automatically marked as defective. Please check the link according to the instructions and then remove this notice.
- ↑ Ishigaki Y, Li X, Serin G, Maquat LE : Evidence for a pioneer round of mRNA translation: mRNAs subject to nonsense-mediated decay in mammalian cells are bound by CBP80 and CBP20 . In: Cell . 106, No. 5, September 2001, pp. 607-17. PMID 11551508 .
- ↑ Balatsos NA, Nilsson P, Mazza C, Cusack S, Virtanen A: Inhibition of mRNA deadenylation by the nuclear cap binding complex (CBC) . In: J. Biol. Chem. . 281, No. 7, February 2006, pp. 4517-22. doi : 10.1074 / jbc.M508590200 . PMID 16317009 .
- ↑ Oh N, Kim KM, Cho H, Choe J, Kim YK: Pioneer round of translation occurs during serum starvation . In: Biochem. Biophys. Res. Commun. . 362, No. 1, October 2007, pp. 145-51. doi : 10.1016 / j.bbrc.2007.07.169 . PMID 17693387 .
