DNA machine

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In biochemistry and biophysics, a DNA machine is a DNA sequence that was designed to fulfill a mechanical function. Designing a DNA machine is a variation on rational design .

properties

The first DNA machine was built in 2000 and functioned as molecular tweezers . When generating a DNA machine - analogous to DNA origami - the hybridization of complementary DNA strands that overlap in sections is used to form three-dimensional structures.

So far, DNA machines have been used for various purposes, e.g. As for the detection of DNA having a detection limit of 0.2  nmol / L ( nanomoles per liter), as contractile DNA sequence for the detection of viral DNA on Gold - nanoparticles , or as a DNA-based catalyst on magnetic particles ( English DNAzymes ). The detection limit for the detection of DNA can be reduced to around 8 fmol / l (femtomol per liter) through various modifications. DNA machines can take on the function of a motor that drives a certain DNA sequence.

See also

Individual evidence

  1. ↑ B. Yurke, AJ Turberfield, AP Mills, FC Simmel, JL Neumann: A DNA-fueled molecular machine made of DNA. In: Nature . Volume 406, Number 6796, August 2000, pp. 605-608, doi : 10.1038 / 35020524 , PMID 10949296 .
  2. ↑ H. Yan, X. Zhang, Z. Shen, NC Seeman: A robust DNA mechanical device controlled by hybridization topology. In: Nature. Volume 415, Number 6867, January 2002, pp. 62-65, doi : 10.1038 / 415062a , PMID 11780115 .
  3. ↑ C. Zhou, Z. Yang, D. Liu: Reversible regulation of protein binding affinity by a DNA machine. In: Journal of the American Chemical Society. Volume 134, Number 3, January 2012, pp. 1416-1418, doi : 10.1021 / ja209590u , PMID 22229476 .
  4. ↑ H. Li, J. Ren, Y. Liu, E. Wang: Application of DNA machine in amplified DNA detection. In: Chemical communications. Volume 50, Number 6, January 2014, pp. 704-706, doi : 10.1039 / c3cc47147k , PMID 24287919 .
  5. ↑ Y. Weizmann, MK Beissenhirtz, Z. Cheglakov, R. Nowarski, M. Kotler, I. Willner: A virus spotlighted by machine of autonomous DNA. In: Angewandte Chemie (International ed. In English). Volume 45, Number 44, November 2006, pp. 7384-7388, doi : 10.1002 / anie.200602754 , PMID 17036292 .
  6. ↑ D. Lubrich, J. Lin, J. Yan: A contractile DNA machine. In: Angewandte Chemie (International ed. In English). Volume 47, number 37, 2008, pp. 7026-7028, doi : 10.1002 / anie.200800476 , PMID 18677787 .
  7. ↑ MK Beissenhirtz, R. Elnathan, Y. Weizmann, I. Willner: The aggregation of Au nanoparticles by an autonomous DNA machine detects viruses. In: Small. Volume 3, Number 3, March 2007, pp. 375-379, doi : 10.1002 / smll.200600450 , PMID 17262868 .
  8. ↑ a b S. Bi, Y. Cui, Y. Dong, N. Zhang: Target-induced self-assembly of DNA nanomachine on magnetic particle for multi-amplified biosensing of nucleic acid, protein, and cancer cell. In: Biosensors & Bioelectronics . Volume 53, March 2014, pp. 207-213, doi : 10.1016 / j.bios.2013.09.066 , PMID 24140870 .
  9. ↑ L. Liu, J. Lei, F. Gao, H. Ju: A DNA machine for sensitive and homogeneous DNA detection via lambda exonuclease assisted amplification. In: Talanta. Volume 115, October 2013, pp. 819-822, doi : 10.1016 / j.talanta.2013.06.062 , PMID 24054668 .
  10. ↑ IK Astakhova, K. Pasternak, MA Campbell, P. Gupta, J. Wengel: A locked nucleic acid-based nanocrawler: designed and reversible movement detected by multicolor fluorescence. In: Journal of the American Chemical Society. Volume 135, Number 7, February 2013, pp. 2423-2426, doi : 10.1021 / ja311250w , PMID 23379691 .