Elastic staining
The elastic staining (also Weigert staining or resorcinol fox staining according to Weigert (1898) ) is a special histological staining for the color-isolated representation of elastic fibers in histological sections.
Coloring principle
With the help of the positively charged dye resorcinol fuchsin (or orcein ), which is offered in excess, it is possible to display the elastic fibers, which are actually difficult to dye, in contrasts due to their dense and gapless packing. By interfaces - adsorption of the positively charged dye of the elastic fibers (superimposed electrostatic polar to the acid and thus negatively charged cladding layer Elastomucin ) to. If desired, to counterstain the other cell components, in particular for representation of the cell nuclei , nuclear fast red , which similarly be used hemalum previously with aluminum sulfate as a mordant was applied.
Reagents
| Resorcinol fuchsin (or orcein ) |
Resorcinol fuchsin contains the basic and positively charged dye diamond fuchsin or orcein in alcoholic solution. |
| Nuclear fast red |
A dye that is mainly related to nuclear staining, but which must first be mixed with a mordant such as alum so that it can fully develop its coloring effect. |
Staining result
| Cores | red |
| Elastic fibers | blue-purple |
| Collagen fibers | pink |
| Musculature | reddish |
literature
- Maria Mulisch, Ulrich Welsch (ed.): Romeis microscopic techniques . 18th edition. Spectrum Akademischer Verlag, Heidelberg 2010, ISBN 978-3-8274-1676-6 , p. 220 .