Embryoid bodies

Embryoid bodies ( EB , for example “embryonic bodies”) are aggregates of cells that arise from embryonic stem cells (ES) in cell culture under certain culture conditions . The phenomenon of the formation of embryoid bodies has been known for decades from research on mouse stem cells.

Cell aggregation to embryoid bodies takes place when stem cells are cultivated without contact with a surface. The aggregates take on a drop-like or round shape and recapitulate the embryonic development of an organism to a limited extent. This is achieved through the differentiation of individual cells into every tissue type of the donor organism with the exception of the trophoectoderm , from which the placenta also arises.

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Individual evidence

↑ EG Berstine, ML Hooper, S. Grandchamp, B. Ephrussi: Alkaline phosphatase activity in mouse teratoma. In: Proc Natl Acad Sci U.S.A. 70 (12), Dec 1973, pp. 3899-3903.
Jump up ↑ A. Leahy, JW Xiong, F. Kuhnert, H. Stuhlmann: Use of developmental marker genes to define temporal and spatial patterns of differentiation during embryoid body formation. In: J Exp Zool. 284 (1), Jun 15, 1999, pp. 67-81.
↑ Gordon M Keller: In vitro differentiation of embryonic stem cells. In: Current Opinion in Cell Biology. 7, 1995, pp. 862-869.

[1] EG Berstine, ML Hooper, S. Grandchamp, B. Ephrussi: Alkaline phosphatase activity in mouse teratoma. In: Proc Natl Acad Sci U.S.A. 70 (12), Dec 1973, pp. 3899-3903.

[2] Jump up ↑ A. Leahy, JW Xiong, F. Kuhnert, H. Stuhlmann: Use of developmental marker genes to define temporal and spatial patterns of differentiation during embryoid body formation. In: J Exp Zool. 284 (1), Jun 15, 1999, pp. 67-81.

[3] Gordon M Keller: In vitro differentiation of embryonic stem cells. In: Current Opinion in Cell Biology. 7, 1995, pp. 862-869.