FLAG tag

The FLAG tag is a protein tag that is used in protein purification and protein characterization of recombinant proteins .

properties

The FLAG tag has the amino acid sequence D Y K DDDDK. This increases the mass by 1012 Da . It can be attached to the N -terminus or the C -terminus of the recombinant protein and also as a TAP tag , but some antibodies only recognize and bind it to the N -terminus, e.g. B. M1 / 4E11. Occasionally the tyrosine in the FLAG tag is sulphated , which can impair antibody binding. As with other protein tags, a proteaseInterface between the recombinant protein and the FLAG tag can be inserted in order to proteolytically cleave off the FLAG tag after purification.

Individual evidence

↑ Thomas P. Hopp, Kathryn S. Prickett, Virginia L. Price, Randell T. Libby, Carl J. March, Douglas Pat Cerretti, David L. Urdal, Paul J. Conlon: A Short Polypeptide Marker Sequence Useful for Recombinant Protein Identification and Purification. In: Bio / Technology. 6, 1988, p. 1204, doi : 10.1038 / nbt1088-1204 .
↑ MR Hunter, NL Grimsey, M. Glass: Sulfation of the FLAG epitope is affected by co-expression of G protein-coupled receptors in a mammalian cell model. In: Scientific reports. Volume 6, June 2016, p. 27316, doi : 10.1038 / srep27316 , PMID 27273047 , PMC 4895180 (free full text).

[Hopp-1] Thomas P. Hopp, Kathryn S. Prickett, Virginia L. Price, Randell T. Libby, Carl J. March, Douglas Pat Cerretti, David L. Urdal, Paul J. Conlon: A Short Polypeptide Marker Sequence Useful for Recombinant Protein Identification and Purification. In: Bio / Technology. 6, 1988, p. 1204, doi : 10.1038 / nbt1088-1204 .

[2] MR Hunter, NL Grimsey, M. Glass: Sulfation of the FLAG epitope is affected by co-expression of G protein-coupled receptors in a mammalian cell model. In: Scientific reports. Volume 6, June 2016, p. 27316, doi : 10.1038 / srep27316 , PMID 27273047 , PMC 4895180 (free full text).