Fluorescence Loss in Photobleaching

As fluorescence loss in photobleaching ( FLIP ) refers to a method of microbiology to detect the fluidity of biological membranes .

functionality

Surface proteins on cells are labeled with fluorescence-marked antibodies and the fluorescence activity is determined photometrically in a defined area . At the same time, there is a spatially separated continuous laser irradiation that destroys the fluorescence in the irradiated area ( see photo bleaching ). As a result, the fluorescence in the measuring range also decreases, which is explained by the mobility of the surface proteins in the membrane ( liquid mosaic model ).

Fluorescence Recovery after Photobleaching ( FRAP ) is based on a similar principle .

Web links

Functional graphs (Major Instruments Co., Ltd., Taiwan) ( Memento from July 17, 2009 in the Internet Archive )

Individual evidence

↑ HC Ishikawa-Ankerhold, R. Ankerhold, GP Drummen: Advanced fluorescence microscopy techniques - FRAP, FLIP, FLAP, FRET and FLIM. In: Molecules . Volume 17, Number 4, 2012, pp. 4047-4132. doi : 10.3390 / molecules17044047 . PMID 22469598 . PDF .
↑ M. Köster, T. Frahm, H. Hauser: Nucleocytoplasmic shuttling revealed by FRAP and FLIP technologies. In: Current Opinion in Biotechnology. Volume 16, Number 1, February 2005, pp. 28-34. doi : 10.1016 / j.copbio.2004.11.002 . PMID 15722012

[1] HC Ishikawa-Ankerhold, R. Ankerhold, GP Drummen: Advanced fluorescence microscopy techniques - FRAP, FLIP, FLAP, FRET and FLIM. In: Molecules . Volume 17, Number 4, 2012, pp. 4047-4132. doi : 10.3390 / molecules17044047 . PMID 22469598 . PDF .

[2] M. Köster, T. Frahm, H. Hauser: Nucleocytoplasmic shuttling revealed by FRAP and FLIP technologies. In: Current Opinion in Biotechnology. Volume 16, Number 1, February 2005, pp. 28-34. doi : 10.1016 / j.copbio.2004.11.002 . PMID 15722012