GYM medium
The GYM medium (abbreviation for G lucose, Y east, and M old - "glucose, yeast, and malt") is a complex nutrient medium for cultivation of bacteria ( bacterial culture ). The medium has proven itself in particular for the cultivation of bacteria of the genus Streptomyces , which is why it is listed and recommended by the DSMZ as a culture medium for Streptomycetes under the name "GYM Streptomyces Medium". It is also known as ISP Medium 2 .
composition
GYM is a complex medium with the following ingredients:
- Glucose (4 g / l)
- Yeast extract (4 g / l)
- Malt extract (10 g / l)
- CaCO 3 (2 g / l, only in solid media)
The pH is adjusted to 7.2. Agar (12 g / l) is added for plate cultures. The medium is autoclaved at 121 ° C. for 20 minutes .
Individual evidence
- ↑ DSMZ: 65th GYM STREPTOMYCES MEDIUM (PDF; 31 kB), accessed on September 15, 2010.
- ↑ bd.com: ISP Medium 1 • ISP Medium 2 • ISP Medium 4 - BD , accessed on July 12, 2015.