Gold sublimate method

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The gold sublimate method is a histopathological procedure for the visualization of glial cells in the central nervous system . It was developed and used by S. Ramón y Cajal at the beginning of the 20th century.

properties

With the gold sublimate method, astrocytes and other macroglia can be displayed well. A special feature of this procedure is that Fañanas cells , which cannot be histologically displayed with any other procedure, are also visible. The process requires pure chemicals. The use of a special gold chloride results in high costs for coloring. The staining solution consists of 0.4 grams of mercury chloride (synonym sublimate ), 10 milliliters of a one percent gold chloride solution and 60 milliliters of water or 0.1% (m / V) gold chloride and 0.5% (m / V) mercury chloride in water.

Globus developed the method for staining already formaldehyde - fixed on Material. With the classic Cajal's method, an addition of bromoformol was also required.

Staining result

The result is a purple-red image in which the background is almost uncolored. The glial cells are visible in a purple-red to brown-red hue. Ganglion cells are only weakly stained.

Individual evidence

  1. ↑ J. Naoumenko, I. Feigin: A modification for paraffin sections of the Cajal gold-sublimate stain for astrocytes. In: Journal of neuropathology and experimental neurology. Volume 20, October 1961, pp. 602-604. PMID 13727746 .
  2. ^ Goodwill Trading Co., Inc .: Histophatologic Techniques. Goodwill Trading Co., Inc., ISBN 978-971-12-0270-5 , pp. 149, 247.
  3. ^ JH Globus: The Cajal and Hortega Glia staining methods. In: Archives of Neurology & Psychiatry. 18, 1927, p. 263, doi: 10.1001 / archneurpsyc.1927.02210020107006 .
  4. ↑ Frederic Roulet: Methods of Pathological Histology . Springer-Verlag, Vienna 1948, p. 428 f .