Gradient electrophoresis
The gradient electrophoresis is a variation of gel electrophoresis or SDS-PAGE , an analytical method of biochemistry for separation of proteins in an electric field . In contrast to gel electrophoresis with a constant polymer content , the gel has a gradually increasing acrylamide content , for example 4-12%. Gels without a polyacrylamide gradient are usually made with 7, 10 or 12% acrylamide. The percentage of a gel determines the molar masses to be optimally separated . The gel solution is prepared with a gradient mixer . The gel is referred to as a gradient gel or gradient gel with transverse pores .
By using gradient gels, a further separation range with regard to the molar mass of very heterogeneous protein mixtures is achieved. Protein mixtures with a lower molar mass can be separated better in 12 percent gel than in 4 percent gel. High molecular weight proteins show poorer separation properties in the 12 percent gel; these are better separated in lower percent gels. A gradient gel represents a compromise that enables samples with small and large proteins to be separated. The staining and documentation is the same as for SDS-PAGE.
literature
- Thomas J. Smith, J. Ellis Bell: An exponential gradient maker for use with minigel polyacrylamide electrophoresis systems . In: Analytical Biochemistry . tape 152 , no. 1 , 1986, pp. 74-77 , doi : 10.1016 / 0003-2697 (86) 90121-1 .
Individual evidence
- ↑ Michael J Dunn: Gel Electrophoresis of Proteins. Elsevier, 2014, ISBN 978-1-483-19354-0 , pp. 97-105.
- ↑ BD Hames: Gel Electrophoresis of Proteins. OUP Oxford, 1998, ISBN 978-0-191-56563-2 , pp. 42-46.