Hanks salts

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As Hanks salts (or salt solution after Hanks or engl. Hanks' BSS (engl. For B alanced S old S olution)) refers to a mixture of different salts (mainly hydrogen carbonates ) used as buffer in media for cell cultures can be used. As a buffer, they guarantee a physiological pH optimum of around 7.0 to 7.4 for cell growth (compare also carbonic acid-bicarbonate system ). It was named after the microbiologist John H. Hanks , who published this buffer solution in 1949. Hanks salts are part of the Balanced Salt Solutions . Hanks salts made from 185.41 mg / l calcium chloride - dihydrate , 48 mg / l disodium hydrogen phosphate , 97.72 mg / l magnesium sulfate , 400 mg / l potassium chloride , 8 g / l sodium chloride , 60 mg / l of potassium dihydrogen phosphate and 1 g / l glucose .

literature

  • R. Ian Freshney: Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications . John Wiley & Sons, 2015, ISBN 978-1-118-87337-3 ( google.com [accessed September 1, 2017]). Page 133
  • Sabine Schmitz: The experimenter: cell culture . Springer-Verlag, 2011, ISBN 978-3-8274-2573-7 ( google.com [accessed September 1, 2017]). Page 122

References and comments

  1. ^ JH Hanks and RE Wallace: Relation of oxygen and temperature in the preservation of tissues by refrigeration , Proc. Soc. Exp. Biol. Med. (1949), Issue 71, Volume 2, pp. 196-200. PMID 18134009 .