Balanced Salt Solution

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A balanced salt solution (BSS) is a solution with a buffered pH value and an isotonic salt concentration.

properties

BSS contain various essential salts in physiological concentrations. As a result, they are basically (i.e., without further additives) iso-osmotic . The osmolality is between 260 and 310 mOsm / kg H 2 O. The pH value is 7.4. Furthermore, they are pyrogen-free and sterile-filtered . Occasionally the pH indicator phenol red (0.015 g / l) or the energy supplier glucose (1 to 4.5 g / l, changes the osmolarity) is added. While maintaining the osmolarity, 5 to 20  mM HEPES can be added to increase the buffer capacity, if in return the concentration of sodium chloride is correspondingly reduced stoichiometrically .

Examples of BSS are the Hanks salts , the Earle salts , the Tyrode solution , the Alsever solution , PBS , TBS , Puck salts , Gey salts , Ringer salts and Simm salts .

BSS can be divided into two groups according to their use: Maintaining the pH value in air (usually phosphate- buffered, e.g. PBS) and maintaining the pH value by fumigation with 5% carbon dioxide (as in a CO 2 incubator , mostly carbonate- buffered, e.g. Earle salts).

Applications

It is used in cell culture for washing or for short-term storage of animal cells and for diluting culture media . They are also used as an isotonic solution in surgery to rinse the operating area.

history

All BSS are derived from Ringer's solution , which was originally developed for heart muscle cells in amphibians. As a direct result, the Tyrode solution was developed for mammalian cells.

Individual evidence

  1. ↑ Gary Gill: Cytopreparation. Springer Science & Business Media, 2012, ISBN 978-1-461-44933-1 , p. 37.
  2. ↑ R. Ian Freshney: Culture of Animal Cells. John Wiley & Sons, 2015, ISBN 978-1-118-87337-3 , p. 133.
  3. ^ Bernice M. Martin: Tissue Culture Techniques. Springer Science & Business Media, 1994, ISBN 978-0-817-63643-2 , p. 199.
  4. ^ PR Yadav: Cell Culture. Discovery Publishing House, 2008, ISBN 978-8-183-56019-1 , p. 166.
  5. ↑ CD Swarna Latha: Cellular Biotechnology. Discovery Publishing House, 2007, ISBN 978-8-183-56198-3 , p. 106.