HEPES

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Structural formula
Structural formula of HEPES
General
Surname 2- (4- (2-Hydroxyethyl) -1-piperazinyl) - ethanesulfonic acid (HEPES)
other names
  • 4- (2-hydroxyethyl) -1-piperazine ethanesulfonic acid
  • N - (2-Hydroxyethyl) piperazine- N ′ - (2-ethanesulfonic acid)
  • HYDROXYETHYLPIPERAZINE ETHANE SULFONIC ACID ( INCI )
Molecular formula C 8 H 18 N 2 O 4 S
Brief description

colorless crystals

External identifiers / databases
CAS number 7365-45-9
EC number 230-907-9
ECHA InfoCard 100,028,098
PubChem 23831
ChemSpider 22278
Wikidata Q418359
properties
Molar mass 238.31 g mol −1
Physical state

firmly

Melting point

210-215 ° C

pK s value
  • 7.77 (4 ° C)
  • 7.48 (25 ° C)
  • 7.39 (37 ° C)
solubility

1350 g l −1 in water (20 ° C)

safety instructions
GHS labeling of hazardous substances
no GHS pictograms
H and P phrases H: no H-phrases
P: no P-phrases
Toxicological data

2000 mg kg −1 ( LD 50 ,  rat ,  oral )

As far as possible and customary, SI units are used. Unless otherwise noted, the data given apply to standard conditions .

HEPES is an abbreviation for 2- (4- (2-hydroxyethyl) -1-piperazinyl) ethanesulfonic acid , a buffer substance .

Chemical properties

HEPES is used for biochemical, molecular biological and microbiological purposes as a buffer substance from the group of Good buffers . With an acid constant of pK s = 7.55 (at 20 ° C) it has a good buffer capacity between pH 6.8 and 8.2. The temperature dependence is ΔpK s / ΔT = −0.014  K −1 .

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  1. ↑ Entry on HYDROXYETHYLPIPERAZINE ETHANE SULFONIC ACID in the CosIng database of the EU Commission, accessed on June 19, 2020.
  2. ↑ a b c d HEPES data sheet (PDF) from Merck , accessed on January 19, 2011.
  3. ↑ a b c d e Good, NE et al. (1966): Hydrogen ion buffers for biological research. In: Biochemistry. Vol. 5, pp. 467-77. PMID 5942950 doi : 10.1021 / bi00866a011
  4. ↑ a b HEPES data sheet , ≥99.5% (titration) from Sigma-Aldrich , accessed on February 15, 2013 ( PDF ).

See also