Alsever's solution

The Alseversche solution is a solution that reduces the clumping of blood cells .

properties

Alsever's solution consists of 2.05% (w / v) dextrose , 0.8% sodium citrate , 0.055% citric acid and 0.42% table salt , as well as the antibiotics neomycin , chloramphenicol and inosine . The citric acid-based buffer binds divalent cations , especially calcium ions , in aqueous solutions with the citrate anion as a chelator . Whole blood samples can be stored at 4 ° C for up to ten weeks with Alsever's solution . An Alsever solution with blood cells is similar in principle to citrated blood .

Applications

The Alseversche solution is used, among other things, in vitro to investigate the anti-inflammatory effect of possible active ingredients and to stabilize blood samples .

history

The first isotonic solution was the Ringer's solution of Sydney Ringer , for storage of living heart muscle cells of amphibians was used. Tyrode then developed the Tyrode solution , which could be used for most mammalian cell types . The Alsever solution was developed in 1941 by John Bellows Alsever and colleagues.

Individual evidence

↑ ^a ^b J. B. Alsever, RB Ainslie: A new method for the preparation of dilute blood plasma and the operation of a complete transfusion service. In: NY State J. Med. 41, 1941, pp. 126-131.
^ DH Campbell, JS Garvey, NE Cremer, DH Sussdorf: Methods in Immunology. A Laboratory Text for Instruction and Research . WA Benjamin, New York 1963, p. 244 .
^ ED Green, BR Curtis, PD Issitt, NS Gutgsell, D. Roelcke, RP Farrar, H. Chaplin: Inhibition of an anti-Pr1d cold agglutinin by citrate present in commercial red cell preservative solutions. In: transfusion. Volume 30, Number 3, Mar-Apr 1990, pp. 267-270, PMID 2316003 .
↑ R. Gandhisan, A. Thamaraichelvan, p Baburaj: Anti-inflammatory action of Lannea coromandelica HRBC membrane stabilization. In: Fitotherapia. 62, 1991, pp. 82-83.
↑ Knud Schjerning-Thiesen: Experiments on the Stability of Sheep Erythrocytes Stored in Alsever's Solution . In: Acta Pathologica Microbiologica Scandinavica . tape 32 , 2009, p. 198 , doi : 10.1111 / j.1699-0463.1953.tb00242.x .
^ LSA, List of CFR Sections Affected . 2003, p. 827 ( google.de ).

[Alsever-1] J. B. Alsever, RB Ainslie: A new method for the preparation of dilute blood plasma and the operation of a complete transfusion service. In: NY State J. Med. 41, 1941, pp. 126-131.

[2] DH Campbell, JS Garvey, NE Cremer, DH Sussdorf: Methods in Immunology. A Laboratory Text for Instruction and Research . WA Benjamin, New York 1963, p. 244 .

[3] ED Green, BR Curtis, PD Issitt, NS Gutgsell, D. Roelcke, RP Farrar, H. Chaplin: Inhibition of an anti-Pr1d cold agglutinin by citrate present in commercial red cell preservative solutions. In: transfusion. Volume 30, Number 3, Mar-Apr 1990, pp. 267-270, PMID 2316003 .

[4] R. Gandhisan, A. Thamaraichelvan, p Baburaj: Anti-inflammatory action of Lannea coromandelica HRBC membrane stabilization. In: Fitotherapia. 62, 1991, pp. 82-83.

[5] Knud Schjerning-Thiesen: Experiments on the Stability of Sheep Erythrocytes Stored in Alsever's Solution . In: Acta Pathologica Microbiologica Scandinavica . tape 32 , 2009, p. 198 , doi : 10.1111 / j.1699-0463.1953.tb00242.x .

[6] LSA, List of CFR Sections Affected . 2003, p. 827 ( google.de ).