Phosphate Buffered Saline Solution
Phosphate -buffered saline (short PBS from English phosphate-buffered saline ) is a buffer solution that is used in biochemistry . The solution contains 137 mM sodium chloride , 2.7 mM potassium chloride and 12 mM total phosphate (in the form of HPO 4 2− and H 2 PO 4 - ). The pH of the adjusted buffer solution is 7.4. The property as a buffer solution enables you to work at this constant pH value. Due to the various salts, the solution has the osmotic pressure of the human organism ( isotonic saline solution ).
application
PBS has many uses because the solution is isotonic and not harmful to cells. It can be used to dilute substances, to purify cell cultures or to extend the lifespan of immobilized biomolecules such as proteins or enzymes . Additives such as EDTA can be added.
composition
One liter of solution contains:
- 8.0 g sodium chloride (NaCl)
- 0.2 g potassium chloride (KCl)
- 1.42 g disodium hydrogen phosphate (Na 2 HPO 4 )
- or
- 1.78 g disodium hydrogen phosphate dihydrate (Na 2 HPO 4 • 2 H 2 O)
- 0.27 g potassium dihydrogen phosphate (KH 2 PO 4 )
Individual evidence
- ↑ Sambrook, J .; Maniatis, T .; Russel, DW: Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press; 3rd edition (2001), ISBN 0-87969-577-3 .