Earle salts

Earle's salts , and Earle's BSS , (Engl. For B alanced S old S olution) in the designated cell culture a buffer . Earle salts are used as an additive to cell culture media in the cell culture of animal cells .

properties

The Earle Salts contain 6.8 g / L sodium chloride , 0.4 g / L potassium chloride , 0.2 g / L calcium chloride , 0.1 g / L magnesium sulfate , 0.14 g / L sodium dihydrogen phosphate , 2.2 g / L sodium hydrogen carbonate and 1 g / L glucose . The pH is then adjusted to 7.2 to 7.3. This is followed by sterile filtration . Like the Hanks salts , the Earle Salts belong to the Balanced Salt Solutions . Since the buffer system is designed for use in a CO ₂ incubator , the pH value increases slowly in air.

history

The Earle Salts were published by Wilton R. Earle and colleagues in 1943 .

literature

• R. Ian Freshney: Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications . John Wiley & Sons, 2015, ISBN 978-1-118-87337-3 . Page 133.
• Sabine Schmitz: The experimenter: cell culture . Springer-Verlag, 2011, ISBN 978-3-8274-2573-7 . Page 122.

Individual evidence

1. ↑ Jay C. Bryant: Earle's balanced salt solution: Preparation of the saline. In: Tissue Culture Association Manual. 1, 1976, p. 185, doi : 10.1007 / BF00916996 .
2. ↑ Wilton R. Earle, Edward L. Schilling, Thomas H. Stark, Nancy P. Straus, Mary F. Brown and Emma Shelton: Production of Malignancy in Vitro. IV. The Mouse Fibroblast Cultures and Changes Seen in the Living Cells. In: JNCI: Journal of the National Cancer Institute. 1943, doi : 10.1093 / jnci / 4.2.165 .