Helicase-dependent amplification

from Wikipedia, the free encyclopedia

The helicase-dependent amplification (HDA, English for 'helicase-dependent amplification') is a method for the amplification (duplication) of DNA . It is a variant of isothermal DNA amplification .

properties

The recombinase polymerase amplification uses a helicase ( TteUvrD , Helicase Superfamily II, from Thermoanaerobacter tengcongensis ), a single-strand-binding protein and a strand-dislocating DNA polymerase . By using a stranded DNA polymerase, the reaction can take place at a constant temperature of 37 to 42 ° C, at room temperature the reaction is somewhat slower. Analogous to qPCR , HDA can also be used to quantify DNA. As with multiplex PCR , several sequences can be duplicated in parallel.

Alternative methods of amplifying DNA are e.g. B. the polymerase chain reaction , the multidisplacement amplification , the isothermal assembly , the loop-mediated isothermal amplification (LAMP), nucleic acid sequence-based amplification ( NASBA ), the recombinase polymerase amplification (RPA), the nicking enzyme amplification reaction (NEAR), the rolling circle replication (RCA). Further detection methods are e.g. B. Nicking endonuclease signal amplification ( NESA ) and nicking endonuclease assisted nanoparticle activation ( NENNA ), exonuclease-aided target recycling , junction or Y-probes , split DNAZyme and deoxyribozyme amplification , non-covalent DNA catalysis and the hybridization chain reaction ( HCR).

Individual evidence

  1. ↑ a b M. Vincent, Y. Xu, H. Kong: helicase-dependent isothermal DNA amplification. In: EMBO reports. Volume 5, number 8, August 2004, ISSN  1469-221X , pp. 795-800, doi: 10.1038 / sj.embor.7400200 , PMID 15247927 , PMC 1249482 (free full text).
  2. ^ Y. Cao, HJ Kim, Y. Li, H. Kong, B. Lemieux: Helicase-dependent amplification of nucleic acids. In: Frederick M. Ausubel et al. (Ed.): Current protocols in molecular biology Volume 104, 2013, ISSN  1934-3647 , S. Unit 15.11, doi: 10.1002 / 0471142727.mb1511s104 , PMID 24510297 .
  3. ↑ J. Kim, CJ Easley: Isothermal DNA amplification in bioanalysis: strategies and applications. In: Bioanalysis. Volume 3, Number 2, January 2011, ISSN  1757-6199 , pp. 227-239, doi: 10.4155 / bio.10.172 , PMID 21250850 .
  4. ↑ L. Lillis, D. Lehman, MC Singhal, J. Cantera, J. Singleton, P. Labarre, A. Toyama, O. Piepenburg, M. Parker, R. Wood, J. Overbaugh, DS Boyle: Non-instrumented incubation of a recombinase polymerase amplification assay for the rapid and sensitive detection of proviral HIV-1 DNA. In: PloS one. Volume 9, number 9, 2014, p. E108189, ISSN  1932-6203 , doi: 10.1371 / journal.pone.0108189 , PMID 25264766 , PMC 4180440 (free full text).
  5. ↑ V. Doseeva, T. Forbes, J. Wolff, Y. Khripin, D. O'Neil, T. Rothmann, I. Nazarenko: Multiplex isothermal helicase-dependent amplification assay for detection of Chlamydia trachomatis and Neisseria gonorrhoeae. In: Diagnostic microbiology and infectious disease. Volume 71, Number 4, December 2011, ISSN  1879-0070 , pp. 354-365, doi: 10.1016 / j.diagmicrobio.2011.08.021 , PMID 22000085 .
  6. ↑ EC Oriero, J. Jacobs, JP Van Geertruyden, D. Nwakanma, U. D'Alessandro: Molecular-based isothermal tests for field diagnosis of malaria and their potential contribution to malaria elimination. In: The Journal of antimicrobial chemotherapy. [electronic publication before printing] September 2014, ISSN  1460-2091 , doi: 10.1093 / jac / dku343 , PMID 25223973 .
  7. ↑ L. Yan, J. Zhou, Y. Zheng, AS Gamson, BT Roembke, S. Nakayama, HO Sintim: Isothermal amplified detection of DNA and RNA. In: Molecular bioSystems. Volume 10, Number 5, May 2014, ISSN  1742-2051 , pp. 970-1003, doi: 10.1039 / c3mb70304e , PMID 24643211 .