Human artificial episomal chromosome

A HAEC ( human artificial episomal chromosome ) is an artificial chromosome that was developed from the replication system of the Epstein-Barr virus and can thus be reproduced in human cells. HAECs were developed in 1994 and are based on the MAC developed by Brown in 1992 .

It serves as a vector and, in contrast to the cosmids, allows larger genome segments to be cloned and is more stable than the YAC . It is a very stable and circular mini-chromosome . To date, insertions of around 330 kbp could be performed with the HAEC.

Individual evidence

^ Sun TQ, Fenstermacher DA, Ves JMH (1994): Human artificial episomal chromosomes for cloning large DNA fragments in human cells , Human Genetics 8 , 33-41
^ JH Bergmann, NM Martins, V. Larionov, H. Masumoto, WC Earnshaw: HACking the centromere chromatin code: insights from human artificial chromosomes. In: Chromosome Res. (2012), Volume 20 (5), pp. 505-19. doi: 10.1007 / s10577-012-9293-0 . PMID 22825423 .

[1] Sun TQ, Fenstermacher DA, Ves JMH (1994): Human artificial episomal chromosomes for cloning large DNA fragments in human cells , Human Genetics 8 , 33-41

[2] JH Bergmann, NM Martins, V. Larionov, H. Masumoto, WC Earnshaw: HACking the centromere chromatin code: insights from human artificial chromosomes. In: Chromosome Res. (2012), Volume 20 (5), pp. 505-19. doi: 10.1007 / s10577-012-9293-0 . PMID 22825423 .