Hybrid plasmid

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The hybrid plasmid is a plasmid that consists of recombinant DNA and is therefore the carrier of its own genetic information and foreign DNA . In a bacterium , the presence of a hybrid plasmid can cause transformation . This creates a transgenic bacterium which can have different properties. For example, a DNA sequence that causes resistance to certain antibiotics is often transported into a bacterium via a hybrid plasmid in order to make it resistant to the active substance.

Manufacturing

isolation

Restriction

The restriction of the foreign DNA (cut out) and the restriction of the plasmid (cut open) are carried out by the same restriction enzyme . The enzyme usually cuts at palindromic sequences, producing so-called sticky ends . The foreign DNA contains the desired gene and is often a carrier of characteristics. However, it can also lead to the override of a sequence already present in the plasmid by being interrupted by the foreign DNA.

Ligation

During ligation , the foreign DNA is connected to the two ends of the cut plasmid by the DNA ligase . This creates the hybrid plasmid.

use

The hybrid plasmid can serve as a vector .

Individual evidence

1. ↑ ^{a b} Genentech Inc. | American corporation. Retrieved December 5, 2019 . 
2. ↑ What is genetic engineering? Retrieved December 5, 2019 . 
3. ↑ biotechnology | Definition, Examples, & Applications. Retrieved December 5, 2019 . 
4. ↑ Recombinant DNA - Genomics. Retrieved December 5, 2019 . 
5. ^ WDR: Gentechnik: Green genetic engineering. September 23, 2019, accessed December 5, 2019 .