Indirect Immunoperoxidase Assay

The indirect immunoperoxidase assay (English: 'indirect immunoperoxidase test', IPA) is a virological method for the detection and quantification of viruses . The IPA is a virological variant of immunohistochemistry in a cell-based immunoassay and was used in virological diagnostics .

principle

The IPA is mainly used for viruses without a cytopathic effect ( non-lytic viruses) to determine a titer . In contrast to lytic viruses, non-lytic viruses cannot be measured in a plaque assay , which is why an IPA or other TCID ₅₀ assays are usually used for this, e.g. B. with the detection of the human coronaviruses SARS -CoV, CoV 229E and CoV OC43, or with the hepatitis A virus . In IPA, cell cultures are inoculated with dilution series of the viruses, after a period of viral replication the cells are fixed , provided with antibodies and detected by immunostaining and counted under a microscope. Instead of an unknown virus concentration, the IPA can also - if the virus concentration is known - be used to determine antibodies, e.g. B. in the human cytomegalovirus or rotaviruses .

Web links

Individual evidence

↑ F. Lambert, H. Jacomy, G. Marceau, PJ Talbot: Titration of human coronaviruses, HcoV-229E and HCoV-OC43, by an indirect immunoperoxidase assay. In: Methods in molecular biology (Clifton, NJ). Volume 454, 2008, ISSN 1064-3745 , pp. 93-102, doi : 10.1007 / 978-1-59745-181-9_8 , PMID 19057861 .
Jump up ↑ F. Lambert, H. Jacomy, G. Marceau, PJ Talbot: Titration of human coronaviruses using an immunoperoxidase assay. In: Journal of visualized experiments: JoVE. Number 14, 2008, ISSN 1940-087X , e751, doi : 10.3791 / 751 , PMID 19066576 , PMC 2582848 (free full text).
↑ EC Nadala, PC Loh: Immunofluorescence and immunoperoxidase assays for the titration of infectious hepatitis A virus (HAV). In: Journal of virological methods. Volume 28, Number 2, May 1990, ISSN 0166-0934 , pp. 117-124, doi : 10.1016 / 0166-0934 (90) 90025-B , PMID 2164520 .
↑ G. Gerna, CJ McCloud, RW Chambers: Immunoperoxidase technique for detection of antibodies to human cytomegalovirus. In: Journal of clinical microbiology. Volume 3, Number 3, March 1976, ISSN 0095-1137 , pp. 364-372, PMID 178690 , PMC 274300 (free full text).
↑ BS Zentner, M. Margalith, A. Galil, B. Halevy, I. Sarov: Detection of rotavirus-specific IgG antibodies by immunoperoxidase assay and enzyme-linked immunosorbent assay. In: Journal of virological methods. Volume 11, Number 3, July 1985, ISSN 0166-0934 , pp. 199-206, PMID 2993335 .

[1] F. Lambert, H. Jacomy, G. Marceau, PJ Talbot: Titration of human coronaviruses, HcoV-229E and HCoV-OC43, by an indirect immunoperoxidase assay. In: Methods in molecular biology (Clifton, NJ). Volume 454, 2008, ISSN 1064-3745 , pp. 93-102, doi : 10.1007 / 978-1-59745-181-9_8 , PMID 19057861 .

[2] Jump up ↑ F. Lambert, H. Jacomy, G. Marceau, PJ Talbot: Titration of human coronaviruses using an immunoperoxidase assay. In: Journal of visualized experiments: JoVE. Number 14, 2008, ISSN 1940-087X , e751, doi : 10.3791 / 751 , PMID 19066576 , PMC 2582848 (free full text).

[3] EC Nadala, PC Loh: Immunofluorescence and immunoperoxidase assays for the titration of infectious hepatitis A virus (HAV). In: Journal of virological methods. Volume 28, Number 2, May 1990, ISSN 0166-0934 , pp. 117-124, doi : 10.1016 / 0166-0934 (90) 90025-B , PMID 2164520 .

[4] G. Gerna, CJ McCloud, RW Chambers: Immunoperoxidase technique for detection of antibodies to human cytomegalovirus. In: Journal of clinical microbiology. Volume 3, Number 3, March 1976, ISSN 0095-1137 , pp. 364-372, PMID 178690 , PMC 274300 (free full text).

[5] BS Zentner, M. Margalith, A. Galil, B. Halevy, I. Sarov: Detection of rotavirus-specific IgG antibodies by immunoperoxidase assay and enzyme-linked immunosorbent assay. In: Journal of virological methods. Volume 11, Number 3, July 1985, ISSN 0166-0934 , pp. 199-206, PMID 2993335 .