Insect cell culture

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The insect cell culture refers to the cell culture of insect cells .

properties

Insect cell cultures are used in biochemistry for basic research and for the production of recombinant proteins . They were originally used to culture insect pathogenic viruses in the 1930s . Baculovirus- based viral vectors are often used as the expression system for the production of recombinant proteins . Typical model organisms from which cell lines for insect cell culture are obtained are z. B. Bombyx mori , Mamestra brassicae , Spodoptera frugiperda ( Sf-9 cells ), Trichoplusia ni and Drosophila melanogaster .

In contrast to the cell culture of mammalian cells , insect cells do not require fumigation with carbon dioxide and lower temperatures; in some cases they grow at room temperature. Furthermore, some of them are less susceptible to human pathogenic contamination , since human pathogenic viruses generally cannot infect insect cells. However, proteins from insect cells have different glycosylations , which can affect the native conformation of a recombinant protein and, in the case of vaccines, its immunogenicity . When using recombinant proteins from insect cell cultures in animals , the glycosylation, which differs from the body's own protein, can lead to immune reactions , e.g. B. in red biotechnology . Therefore, through pathway design, transgenic insect cell lines are being developed whose glycosylation patterns are more similar to human glycosylation patterns.

application

Some vaccines have been produced in insect cell cultures and purified by protein purification , e.g. B. Cervarix .

Individual evidence

  1. ↑ Sabine Schmitz: The experimenter: cell culture . Spectrum Academic Publishing House, 2011, ISBN 9783827425720 .
  2. ↑ B. Arif, L. Pavlik: Insect cell culture: virus replication and applications in biotechnology. In: Journal of invertebrate pathology. Volume 112 Suppl, March 2013, ISSN  1096-0805 , pp. S138-S141, doi : 10.1016 / j.jip.2012.07.011 , PMID 22841637 .
  3. ↑ S. Sokolenko, S. George, A. Wagner, A. Tuladhar, JM Andrich, MG Aucoin: Co-expression vs. co-infection using baculovirus expression vectors in insect cell culture: Benefits and drawbacks. In: Biotechnology Advances . Volume 30, number 3, 2012 May-Jun, ISSN  1873-1899 , pp. 766-781, doi : 10.1016 / j.biotechadv.2012.01.009 , PMID 22297133 .
  4. ↑ JC Drugmand, YJ Schneider, SN Agathos: Insect cells as factories for biomanufacturing. In: Biotechnology Advances . Volume 30, number 5, 2012 Sep-Oct, ISSN  1873-1899 , pp. 1140-1157, doi : 10.1016 / j.biotechadv.2011.09.014 , PMID 21983546 .
  5. ^ WF Hink: Established insect cell line from the cabbage looper, Trichoplusia ni. In: Nature . Volume 226, Number 5244, May 1970, pp. 466-467, doi : 10.1038 / 226466b0 , PMID 16057320 .
  6. ↑ GL Zheng, HX Zhou, CY Li: Serum-free culture of the suspension cell line QB-Tn9-4s of the cabbage looper, Trichoplusia ni, is highly productive for virus replication and recombinant protein expression. In: Journal of insect science. Volume 14, February 2014, p. 24, doi : 10.1093 / jis / 14.1.24 , PMID 25373171 , PMC 4199540 (free full text).
  7. ↑ T. Katoh, M. Tiemeyer: The N's and O's of Drosophila glycoprotein glycobiology. In: Glycoconjugate journal. Volume 30, number 1, January 2013, ISSN  1573-4986 , pp. 57-66, doi : 10.1007 / s10719-012-9442-x , PMID 22936173 , PMC 3548036 (free full text).
  8. ^ A. Contreras-Gómez, A. Sánchez-Mirón, F. García-Camacho, E. Molina-Grima, Y. Chisti: Protein production using the baculovirus-insect cell expression system. In: Biotechnology progress. Volume 30, number 1, 2014 Jan-Feb, ISSN  1520-6033 , pp. 1-18, doi : 10.1002 / btpr.1842 , PMID 24265112 .
  9. ↑ B. Schiller, A. Hykollari, S. Yan, K. Paschinger, IB Wilson: Complicated N-linked glycans in simple organisms. In: Biological chemistry. Volume 393, number 8, August 2012, ISSN  1437-4315 , pp. 661-673, doi : 10.1515 / hsz-2012-0150 , PMID 22944671 , PMC 3589692 (free full text).
  10. ^ JA Mena, AA Kamen: Insect cell technology is a versatile and robust vaccine manufacturing platform. In: Expert review of vaccines. Volume 10, Number 7, July 2011, ISSN  1744-8395 , pp. 1063-1081, doi : 10.1586 / erv.11.24 , PMID 21806400 .