Integrase
| Integrase | ||
|---|---|---|
|
||
| 2-domain integrase of the Rous sarcoma virus. | ||
| Enzyme classification | ||
| EC, category | 2.7.7. , Nucleotidyl transferase | |
| Substrate | DNA | |
The integrase is an enzyme of retroviruses that is responsible for the integration of viral DNA strands into the chromosomes of the host cell . It is one of three key retrovirus enzymes. The integrase is a nucleotidyltransferase, by the pol - gene of the virus RNA is coded. As an endonuclease after reverse transcription, it can split the host cell DNA and integrate the virus genome into the host DNA.
integration
The integrase binds to the viral DNA and to the host DNA and forms a complex with them. The figure shows the sequence of the DNA strand breaks and the subsequent composition when integrating the viral DNA. The integrase monomers are shown in gray, the viral DNA in red and the host DNA in black, and the red dots the 5 'ends of the viral DNA.
see also: Integration in retroviruses
HI virus
The most well-studied integrase of HIV is a 32 kDa large protein , which when released as antigen acts. HIV integrase is the target of therapeutic approaches to the treatment of HIV by integrase inhibitors . These include Raltegravir (also known as MK-0518 known), elvitegravir and since 2014 approved in Europe Dolutegravir .
literature
Morales-Ramirez JO, Teppler H, Kovacs C, et al. Antiretroviral effect of MK-0518, a novel HIV-1 integrase inhibitor, in ART-naïve HIV-1 infected patients. Program and abstracts of the 10th European AIDS Conference; November 17-20, 2005; Dublin, Ireland. Abstract LBPS1 / 6.