Cold grinding

How cold grinding works by means of a solenoid .

Under a cold grinding ( English Cryogenic grinding ) meant the milling substances which have previously been strongly cooled.

For example, thermoplastics are difficult to grind to small particle sizes because they become soft at room temperature and clump together when grinded. If they are cooled to −196 ° C by dry ice , liquid carbon dioxide or liquid nitrogen , these plastics can be ground in powder form. Plant and animal tissue can also be finely ground for microbiological use. A number of recent studies report on the processing and behavior of nanostructured materials by cold milling.

Cold milling can be used to destroy cells, which is useful for protein extraction. This allows the fine powder to be stored at −80ºC for a long time without any apparent change in its biochemical properties.

Forensic dentistry

In the context of forensic dentistry , teeth can be used to extract deoxyribonucleic acid (DNA) from the pulp (the tooth pulp , popularly the "nerve") to establish identity , after the pulp is protected by the surrounding tooth enamel and dentin as if by armor . For this purpose, the pulp cavity can be opened and the pulp removed. Since the risk of contamination is very high, the tooth has recently been grinded by means of cold grinding and the DNA is extracted from the powder for DNA analysis using the polymerase chain reaction .

Individual evidence

1. ↑ Michal Domanski, Kelly Molloy, Improved methodology for the affinity isolation of human protein complexes expressed at near endogenous levels ( Memento of the original from March 31, 2013 in the Internet Archive ) Info: The archive link was inserted automatically and has not yet been checked. Please check the original and archive link according to the instructions and then remove this notice. , BioTechnics. Retrieved March 13, 2017. 
2. ^ DNA and odontology , ForensicDentistryOnline. Retrieved March 13, 2017.
3. ↑ Catherine Adams, Romina Carabott, Sam Evans: Forensic Odontology: An Essential Guide . Wiley, November 11, 2013, ISBN 978-1-118-52613-2 , pp. 113-117.