Kimmig agar

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The Kimmig agar or mushroom agar according to Kimmig is a nutrient medium that is suitable , among other things, for growing mushrooms due to its low pH value . The Kimmig agar was first described by Joseph Kimmig in 1953 . In particular, it promotes the growth of conidia . It is used to identify fungal contamination and as a ( selective ) nutrient medium for cultivating fungi. The formation of a cotton wool-like mycelium is largely prevented.

properties

The Kimmig agar is usually clear and light yellow in color. Its pH is 6.5 ± 0.2. The firmness is similar to that of a 1.5% agar gel . Fungal cultures of Aspergillus brasiliensis ( ATCC 16404; formerly A. niger ), Candida albicans (ATCC 10231), Saccharomyces cerevisiae (ATCC 9763), Trichophyton mentagrophytes (ATCC 18748), as well as related species should grow very well to moderately on Kimmig agar.

Manufacture and composition

In the preparation are usually peptone and casein , bringing the nitrogen-containing nutrients in the solution, glucose , which serves as a source of carbohydrate, agar-agar and sodium chloride used to maintain the osmotic balance. The ingredients are added to a 0.5% glycerine solution and heated. The agar can be made selective by adding antibiotics such as penicillin , streptomycin , colistin and novobiocin . As a result, numerous bacteria can no longer grow on the medium. An addition of cycloheximide (Actidion) can be used to inhibit the growth of yeast .

Individual evidence

  1. ↑ Frauke Ilse-Marie Sareika: Evaluation of various examination methods in onychomycosis diagnostics: native diagnostics and culture findings compared to the microscopic assessment of PAS-stained sections. (PDF) In: Dissertation. 2010, accessed May 13, 2018 .
  2. ↑ JU Kimmig, H. Riech: Antimykotica in Experiment and Klinik . In: drug research . No. 3 , 1953, pp. 267-276 .
  3. ↑ a b mushroom agar according to KIMMIG. (PDF) (No longer available online.) Noll Dressing Materials, March 26, 2007, archived from the original on May 14, 2018 ; accessed on May 13, 2018 . Info: The archive link was inserted automatically and has not yet been checked. Please check the original and archive link according to the instructions and then remove this notice. @1@ 2Template: Webachiv / IABot / noll-verbandstoffe.de
  4. ↑ Ulbricht, Horst .: Human pathogenic fungi of the skin and mucous membranes: removal, cultivation, differentiation . Schlueter, Hannover 1999, ISBN 3-87706-540-6 .
  5. ↑ a b Fungi Kimmig Agar Base. (PDF) Himedia, February 2015, accessed on May 13, 2018 .
  6. ↑ Detlef Hantschke: A Colistin-Novobiocin-Actidion-Agar as a cultivation medium for human pathogenic fungi . In: Mycoses . tape 11 , no. November 11 , 1968, ISSN  0933-7407 , p. 769-778 , doi : 10.1111 / j.1439-0507.1968.tb03303.x .
  7. ↑ Georg LK, Ajello L. and Papageorge C .: Use of cycloheximide in the selective isolation of fungi pathogenic to man . In: J. Lab. Clin. Med. No. 44 , 1954, pp. 422 .