Confluence (cell culture)

Snu449 cells at around 50 to 60% confluence.

Snu449 cells at around 100% confluence.

When confluency largely complete coverage refers to the surface of a culture vessel with adherent (adherent) cells . Confluence varies from cell line to cell line.

At confluence, the cells are in very close cell-cell contact , so that no surface is freely available as a substrate . Most cells stop dividing because of contact inhibition .

In a continuous cell culture of adherent cell lines a separating and implementation should the cells (engl. Splitting , passengers subculture ', coll., Split') are carried out before the complete confluence was reached, the through contact inhibition produced slowing the rate of cell division to avoid. Trypsin can also be used for this, which detaches the cells from the surface by cleaving extracellular proteins.

literature

Sabine Schmitz: The experimenter: cell culture. Third edition. Spektrum Akademischer Verlag, Heidelberg 2011, ISBN 3-8274-2572-7 .

Individual evidence

↑ Hsiang-Ling Huang, Hsiang-Wei Hsing, Tzu-Chia Lai, Yi-Wen Chen, Tian-Ren Lee: Trypsin-induced proteome alteration during cell subculture in mammalian cells . In: Journal of Biomedical Science . tape 17 , no. 1 , May 11, 2010, ISSN 1021-7770 , p. 36 , doi : 10.1186 / 1423-0127-17-36 , PMID 20459778 , PMC 2873939 (free full text).

[1] Hsiang-Ling Huang, Hsiang-Wei Hsing, Tzu-Chia Lai, Yi-Wen Chen, Tian-Ren Lee: Trypsin-induced proteome alteration during cell subculture in mammalian cells . In: Journal of Biomedical Science . tape 17 , no. 1 , May 11, 2010, ISSN 1021-7770 , p. 36 , doi : 10.1186 / 1423-0127-17-36 , PMID 20459778 , PMC 2873939 (free full text).