L -ascorbate oxidase
| L-ascorbate oxidase | ||
|---|---|---|
| other names |
Ascorbic acid oxidase |
|
| Precursor | 578 amino acids , 64,865 Da | |
| Identifier | ||
| External IDs | ||
| Enzyme classification | ||
| EC, category | 1.10.3.3 | |
| Orthologue ( Brassica rapa ) | ||
| Entrez | 103845610 | |
| UniProt |
Q40588
|
|
| PubMed search |
103845610
|
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L- ascorbate oxidase is an enzyme and membrane protein belonging to the group of oxidases .
properties
The L ascorbate oxidase comes in plants against, inter alia, in turnip rape ( Brassica rapa ), in Pumpkin ( Cucurbita maxima ), in the zucchini ( Cucurbita pepo var. Melopepo ) in Cucumber ( Cucumis sativus ), and in the tobacco plant ( Nicotiana tabacum ). In the redox metabolism , it catalyzes the oxidation of ascorbic acid :
The L ascorbate oxidase is glycosylated and has disulfide bridges . Copper ions are cofactors of L -ascorbate oxidase. It is believed to be more involved in plant growth than in a stress response to ozone , salts , drought and phytopathogens . The enzyme activity of L -ascorbate oxidase can be measured photometrically at a wavelength of 245 nm.
Individual evidence
- ↑ AAO - L-ascorbate oxidase precursor - Nicotiana tabacum (Common tobacco) - AAO gene & protein. In: uniprot.org. December 5, 2018, accessed February 17, 2019 .
- ↑ N. Smirnoff: Ascorbic acid metabolism and functions: A comparison of plants and mammals. In: Free radical biology & medicine. Volume 122, July 2018, pp. 116–129, doi : 10.1016 / j.freeradbiomed.2018.03.033 , PMID 29567393 , PMC 6191929 (free full text).
- ↑ Enzymatic Assay of ASCORBATE OXIDASE ( Memento from June 26, 2011 in the Internet Archive ) In: SigmaAldrich.com. (PDF; 18 kB).