Liposome production

from Wikipedia, the free encyclopedia

The liposomes generating comprises methods for the production of liposomes .

properties

The different liposome shapes can be produced by different methods. If a biocatalyst is surrounded by a liposome, the process is called membrane separation , which is a form of immobilization .

Multilamellar liposomes (MLV) are formed spontaneously by dissolving lipids in the aqueous phase. Unilamellar liposomes (SUV / LUV) can pass through

  • slow dropping of ethanolic or ethereal lipid solutions into the aqueous phase,
  • Extrusion of MLV through polycarbonate membranes of a certain pore size (e.g. 100 nm),
  • Dialysis of detergent- lipid mixtures or
  • Ultrasound and vibration of aqueous solutions on previously dried lipid films

produce.

The choice of the manufacturing method depends on several factors:

The formation of liposomes is not a spontaneous process, but takes place through the addition of phospholipids to aqueous liquids and the supply of energy. Low shear forces produce MLV, while uniform, high shear forces produce smaller ULV with roughly equal proportions of lipids and entrapped materials. Ultrasound can damage the substances to be enclosed.

literature

  • RRC New (Ed.): Liposomes a practical approach. IRL Press at Oxford University Press, Oxford 1990, ISBN 0-19-963077-1 .
  • Dietrich Arndt, Iduna Fichtner: Liposomes: Presentation - Properties - Application. Akademie-Verlag, Berlin 1986, ISBN 3-05-500148-6 . (Advances in Oncology, Vol. 13)
  • Nejat Düzgünes: Liposomes, Part G . In: Methods in Enzymology , Humana 2009, ISBN 978-0-12-381379-4 .
  • Volker Weissig (Ed.): Liposomes: Methods and Protocols. Volume 1: Pharmaceutical nanocarriers. Humana Press, New York 2010, ISBN 978-1-60327-360-2 . (Methods in Molecular Biology, Volume 605)
  • Volker Weissig (Ed.): Liposomes: Methods and Protocols. Volume 2: Biological Membrane Models. Humana Press, New York 2010, ISBN 978-1-607-61446-3 . (Methods in Molecular Biology, Volume 606)
  • Shelley D. Minteer (Ed.): Enzyme Stabilization and Immobilization: Methods and Protocols. Humana Press, Totowa NJ 2011, ISBN 978-1-60761-895-9 . (Methods in Molecular Biology, Volume 679)
  • S. Simões, JN Moreira, C. Fonseca, N. Düzgünes, MC de Lima: On the formulation of pH-sensitive liposomes with long circulation times. In: Advanced Drug Delivery Reviews . Volume 56, Number 7, April 2004, pp. 947-965, ISSN  0169-409X . doi : 10.1016 / j.addr.2003.10.038 . PMID 15066754 .

Individual evidence

  1. ^ NF Morales-Penningston, J. Wu, ER Farkas, SL Goh, TM Konyakhina, JY Zheng, WW Webb, GW Feigenson: GUV preparation and imaging: minimizing artifacts. In: Biochimica et Biophysica Acta . Volume 1798, Number 7, July 2010, pp. 1324-1332, ISSN  0006-3002 . doi : 10.1016 / j.bbamem.2010.03.011 . PMID 20302841 . PMC 2885611 (free full text).
  2. ↑ MR Mozafari: Liposomes: an overview of manufacturing techniques. In: Cellular & molecular biology letters. Volume 10, Number 4, 2005, pp. 711-719, ISSN  1425-8153 . PMID 16341279 . PDF .
  3. ^ A. Gomez-Hens, JM Fernandez-Romero: Analytical methods for the control of liposomal delivery systems. In: Trends Anal Chem . 25, 2006, pp. 167-178.
  4. ↑ MR Mozafari, C. Johnson, S. Hatziantoniou, C. Demetzos: Nanoliposomes and their applications in food nanotechnology. In: Journal of Liposome Research . (2008), Volume 18 (4), pp. 309-327.
  5. ↑ MR Mozafari, SM Mortazavi: Nanoliposomes: From Fundamentals to Recent Developments. Trafford Publishing, Oxford 2005, UK.
  6. ↑ JC Colas, WL Shi, VSNM Rao, A. Omri, MR Mozafari, H. Singh: Microscopical investigations of nisin-loaded nanoliposomes prepared by Mozafari method and their bacterial targeting. In: Micron. (2007), Volume 38, pp. 841-847.