Neoschizomer
As Neoschizomere in the are molecular biology pairs or groups of restriction enzymes indicated that specifically the same nucleotide sequence recognized, but these columns in different ways. They produce indistinguishable fragments on restriction digestion because the restriction sites are close to each other.
| enzyme | source | Recognition sequence | Restriction cut | end up |
|---|---|---|---|---|
| Sma I | Serratia marcescens |
5'-CCCGGG-3' 3'-GGGCCC-5' |
5'-CCC GGG-3' 3'-GGG CCC-5' |
no overhang ( smooth ends ) |
| Xma I | Xanthomonas malvacearum |
5'-C CCGGG-3' 3'-GGGCC C-5' |
5 'overhang with four bases ( sticky ends ) |
Further examples of pairs of neoschizomers are Kpn I and Acc 65I, Sac I and Eco ICRI, Aat I and Zra I. Apart from the identity of the recognition sequence, there are often no major similarities between neoschizomers in terms of amino acid sequence and structure. These enzymes are isolated from different bacterial strains and often have different requirements for optimal restriction digestion.
See also
literature
- Friedrich Lottspeich , Haralabos Zorbas: Bioanalytics . Spektrum Akademischer Verlag, Heidelberg 1998, ISBN 978-3-8274-0041-3 .
- Cornel Mülhardt: The Experimenter: Molecular Biology / Genomics. Sixth edition. Spectrum Akademischer Verlag, Heidelberg 2008, ISBN 3-8274-2036-9 .
- J. Sambrook , T. Maniatis , DW Russel: Molecular cloning: a laboratory manual. 3rd edition (2001), Cold Spring Harbor Laboratory Press. ISBN 0-87969-577-3 .