P1 Artificial Chromosomes

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A P1 artificial chromosome ( PAC ) (also P1-derived or P1-based) is an artificial chromosome which was developed from the P1 vector of the temperate bacteriophage P1. In contrast to the cosmids , artificial chromosomes allow cloning of larger genome segments . PAC serves as a vector and is more stable than the YAC .

The first PAC was developed by Ioannou (see literature) using a combination of the P1 vector and the F-factor system and can be used for insertions of genome segments with lengths between 100 and 300 kbp .

literature

  • PA Ioannou, CT Amemiya, J. Garnes, PM Kroisel, H. Shiyuza, C. Chen, MA Batzer, PJ de Jong: A new bacteriphage P1-derived vector for the propagation of large human DNA fragments. In: Nature Genetics . 6, 1994, pp. 84-89.

See also