Protein L

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Protein L (blue) on the light chain of a murine antibody

The protein L is a protein from Peptostreptococcus magnus , the antibody binds.

properties

Protein L is used for immune evasion and is a virulence factor . The binding of antibodies reduces an antigen-antibody reaction . Protein L has 719 amino acids and has low homology to other bacterial antibody-binding proteins such as Protein A , Protein G , Protein A / G or M protein . Protein L binds to the variable region of the light chains (in the region of VκI, VκIII and VκIV, but not VκII) of immunoglobulins of the IgG, IgM, IgA, IgE and IgD types, as well as scFv .

Individual evidence

  1. ↑ L. Björck: Protein L. A novel bacterial cell wall protein with affinity for Ig L chains. In: Journal of Immunology (Baltimore, Md.: 1950). Volume 140, Number 4, February 1988, pp. 1194-1197, PMID 3125250 .
  2. ↑ BH Nilson, L. Lögdberg, W. Kastern, L. Björck, B. Akerström: Purification of antibodies using protein L-binding framework structures in the light chain variable domain. In: Journal of immunological methods. Volume 164, Number 1, August 1993, pp. 33-40, PMID 8360508 .
  3. ↑ M. Graille, S. Harrison, MP Crump, SC Findlow, NG Housden, BH Muller, N. Battail-Poirot, G. Sibai, BJ Sutton, MJ Taussig, C. Jolivet-Reynaud, MG Gore, EA Stura: Evidence for plasticity and structural mimicry at the immunoglobulin light chain-protein L interface. In: The Journal of biological chemistry. Volume 277, Number 49, December 2002, pp. 47500-47506, doi : 10.1074 / jbc.M206105200 , PMID 12221088 .
  4. ↑ Y. Safdari, p Farajnia, M. Asgharzadeh, SA Khosroshahi, K. Veisi, V. Ahmadzadeh, M. Khalili: Protein L: a robust enzyme-conjugated molecule for detection of humanized single chain antibodies. In: Monoclonal antibodies in immunodiagnosis and immunotherapy. Volume 32, number 6, December 2013, pp. 409-412, doi : 10.1089 / mab.2013.0049 , PMID 24328745 .