Protein A / G

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The protein A / G is an artificial fusion protein from the bacterial antibodies -binding proteins A and G .


Protein A / G, like the related proteins A or G, is used in biochemistry for protein purification and the measurement of antibodies. Protein A / G is a partially has homology to other bacterial antibody-binding proteins such as Protein A , Protein G , but only little similarity to protein L or M protein . Protein A / G binds to the heavy chains of human immunoglobulins of the types IgA, IgE, IgM and, to a lesser extent, IgD. IgA and IgD are not bound among mouse immunoglobulins. The proteins A and G also bind to the DNA-binding proteins Ku and PARP-1 .

Individual evidence

  1. Jump up RK Grover, X. Zhu, T. Nieusma, T. Jones, I. Boero, AS MacLeod, A. Mark, S. Niessen, HJ Kim, L. Kong, N. Assad-Garcia, K. Kwon, M. Chesi, VV Smider, DR Salomon, DF Jelinek, RA Kyle, RB Pyles, JI Glass, AB Ward, IA Wilson, RA Lerner: A structurally distinct human mycoplasma protein that generically blocks antigen-antibody union. In: Science. Volume 343, number 6171, February 2014, pp. 656-661, doi : 10.1126 / science.1246135 , PMID 24503852 , PMC 3987992 (free full text).
  2. B. Heelan: Purification of monoclonal antibodies using protein a / g. In: Methods in molecular medicine. Volume 40, 2000, pp. 281-288, doi : 10.1385 / 1-59259-076-4: 281 , PMID 21337096 .
  3. OP Dancette, JL Taboureau, E. Tournier, C. Charcosset, P. Blond: Purification of immunoglobulins G by protein A / G affinity membrane chromatography. In: Journal of chromatography. B, Biomedical Sciences and Applications. Volume 723, Number 1-2, February 1999, pp. 61-68, PMID 10080633 .
  4. L. Lu, Y. Wang: Immunoprecipitation alert: DNA binding proteins directly bind to protein A / G without any antibody as the bridge. In: Cell cycle (Georgetown, Tex.). Volume 7, Number 3, February 2008, pp. 417-418, PMID 18235234 .