Northern reverse blot

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The reverse northern blot is a molecular biological method in which the expression of several genes can be examined simultaneously with labeled cDNA .

The name is derived from the Northern Blot , in which RNA isolated from tissue-specific cells is transferred to a membrane after gel electrophoresis in order to then detect the expression of this gene with a specific gene probe . The reverse northern blot also isolates the RNA, but serves as a template for an RT-PCR . Using radioactive or modified nucleotides creates a cDNA probe. This is used to hybridize membranes to which PCR products or cloned cDNA have been applied beforehand ("DNA spot") and denatured as in the Southern blot . Positive signals now indicate whether the corresponding gene is expressed in the examined tissue. The method can be seen as the predecessor of DNA chip technology .

Individual evidence

  1. Daniel W Dilks, Robert H Ring, Xavier Z Khawaja, Thomas J Novak, Christopher Aston: High-throughput confirmation of differential display PCR results using reverse Northern blotting . In: Journal of Neuroscience Methods . 123, No. 1, 2003, ISSN  0165-0270 , pp. 47-54. doi : 10.1016 / S0165-0270 (02) 00343-6 .

See also