Northern reverse blot
The name is derived from the Northern Blot , in which RNA isolated from tissue-specific cells is transferred to a membrane after gel electrophoresis in order to then detect the expression of this gene with a specific gene probe . The reverse northern blot also isolates the RNA, but serves as a template for an RT-PCR . Using radioactive or modified nucleotides creates a cDNA probe. This is used to hybridize membranes to which PCR products or cloned cDNA have been applied beforehand ("DNA spot") and denatured as in the Southern blot . Positive signals now indicate whether the corresponding gene is expressed in the examined tissue. The method can be seen as the predecessor of DNA chip technology .
- Daniel W Dilks, Robert H Ring, Xavier Z Khawaja, Thomas J Novak, Christopher Aston: High-throughput confirmation of differential display PCR results using reverse Northern blotting . In: Journal of Neuroscience Methods . 123, No. 1, 2003, doi : 10.1016 / S0165-0270 (02) 00343-6 . , pp. 47-54.