RiAFP

RiAFP (H4)

RiAFP is a group of anti-freeze proteins (AFP) from the longhorn beetle Rhagium inquisitor .

properties

The six RiAFPs described so far have a mass of about 12.5 to 12.8 kilodaltons , an isoelectric point above eight and several repetitions of the amino acid sequence Tx TxTxT at irregular intervals. RiAFP (H4) has one of the most regular protein structures of all proteins examined so far. RiAFP still have no homologies to known proteins.

The anti-freeze proteins from beetles are among those with the strongest thermal hysteresis effect , with Tenebrio molitor it is about a hundred times stronger than with the anti-freeze proteins from polar fish and, at a concentration of 1 mg / ml, leads to a reduction in the freezing point of 5 , 5 ° C.

Web links

PDB 4DT5 - Structure of RiAFP (H4)

Individual evidence

↑ E. Kristiansen, H. Ramløv, L. Hagen, SA Pedersen, RA Andersen, KE Zachariassen: Isolation and characterization of hemolymph antifreeze proteins from larvae of the longhorn beetle Rhagium inquisitor (L.). In: Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology. Volume 142, Number 1, September 2005, ISSN 1096-4959 , pp. 90-97, doi : 10.1016 / j.cbpc.2005.06.004 , PMID 15993638 .
↑ E. Kristiansen, H. Ramløv, P. Højrup, SA Pedersen, L. Hagen, KE Zachariassen: Structural characteristics of a novel antifreeze protein from the longhorn beetle Rhagium inquisitor. In: Insect biochemistry and molecular biology. Volume 41, Number 2, February 2011, ISSN 1879-0240 , pp. 109-117, doi : 10.1016 / j.ibmb.2010.11.002 , PMID 21078390 .
↑ A. Hakim, JB Nguyen, K. Basu, DF Zhu, D. Thakral, PL Davies, FJ Isaacs, Y. Modis, W. Meng: Crystal structure of an insect antifreeze protein and its implications for ice binding. In: The Journal of biological chemistry. Volume 288, Number 17, April 2013, ISSN 1083-351X , pp. 12295-12304, doi : 10.1074 / jbc.M113.450973 , PMID 23486477 , PMC 3636913 (free full text).
↑ A. Hakim, D. Thakral, DF Zhu, JB Nguyen: Expression, purification, crystallization and preliminary crystallographic studies of Rhagium inquisitor antifreeze protein. In: Acta crystallographica. Section F, Structural biology and crystallization communications. Volume 68, Pt 5 May 2012, ISSN 1744-3091 , pp. 547-550, doi : 10.1107 / S1744309112010421 , PMID 22691785 , PMC 3374510 (free full text).
↑ Graham LA, Liou YC, Walker VK, Davies PL: Hyperactive antifreeze protein from beetles. In: Nature. Volume 388, Number 6644, August 1997, ISSN 0028-0836 , pp. 727-728, doi : 10.1038 / 41908 , PMID 9285581 .

[1] E. Kristiansen, H. Ramløv, L. Hagen, SA Pedersen, RA Andersen, KE Zachariassen: Isolation and characterization of hemolymph antifreeze proteins from larvae of the longhorn beetle Rhagium inquisitor (L.). In: Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology. Volume 142, Number 1, September 2005, ISSN 1096-4959 , pp. 90-97, doi : 10.1016 / j.cbpc.2005.06.004 , PMID 15993638 .

[2] E. Kristiansen, H. Ramløv, P. Højrup, SA Pedersen, L. Hagen, KE Zachariassen: Structural characteristics of a novel antifreeze protein from the longhorn beetle Rhagium inquisitor. In: Insect biochemistry and molecular biology. Volume 41, Number 2, February 2011, ISSN 1879-0240 , pp. 109-117, doi : 10.1016 / j.ibmb.2010.11.002 , PMID 21078390 .

[3] A. Hakim, JB Nguyen, K. Basu, DF Zhu, D. Thakral, PL Davies, FJ Isaacs, Y. Modis, W. Meng: Crystal structure of an insect antifreeze protein and its implications for ice binding. In: The Journal of biological chemistry. Volume 288, Number 17, April 2013, ISSN 1083-351X , pp. 12295-12304, doi : 10.1074 / jbc.M113.450973 , PMID 23486477 , PMC 3636913 (free full text).

[4] A. Hakim, D. Thakral, DF Zhu, JB Nguyen: Expression, purification, crystallization and preliminary crystallographic studies of Rhagium inquisitor antifreeze protein. In: Acta crystallographica. Section F, Structural biology and crystallization communications. Volume 68, Pt 5 May 2012, ISSN 1744-3091 , pp. 547-550, doi : 10.1107 / S1744309112010421 , PMID 22691785 , PMC 3374510 (free full text).

[PMID_9285581-5] Graham LA, Liou YC, Walker VK, Davies PL: Hyperactive antifreeze protein from beetles. In: Nature. Volume 388, Number 6644, August 1997, ISSN 0028-0836 , pp. 727-728, doi : 10.1038 / 41908 , PMID 9285581 .